Mouse TIGIT Protein 4157

$270.00 – $900.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Gly26-Thr143

SKU: 4157parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001139797
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
molecular weight	The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse TIGIT Protein 4157

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Protein names GTPase NRas (EC 3.6.5.2) (Transforming protein N-Ras)
Gene names NRAS,NRAS HRAS1
Protein family Small GTPase superfamily, Ras family
Mass 21229Da
Function FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. {ECO:0000269\|PubMed:30712867}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2; Evidence={ECO:0000250\|UniProtKB:P01116};
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:15705808, ECO:0000269\|PubMed:26701913}; Lipid-anchor {ECO:0000269\|PubMed:15705808, ECO:0000269\|PubMed:26701913}; Cytoplasmic side {ECO:0000269\|PubMed:15705808, ECO:0000269\|PubMed:26701913}. Golgi apparatus membrane {ECO:0000269\|PubMed:15705808, ECO:0000269\|PubMed:26701913}; Lipid-anchor {ECO:0000269\|PubMed:15705808, ECO:0000269\|PubMed:26701913}. Note=Shuttles between the plasma membrane and the Golgi apparatus. {ECO:0000269\|PubMed:15705808, ECO:0000269\|PubMed:26701913}.
Structure SUBUNIT: Interacts (active GTP-bound form preferentially) with RGS14 (By similarity). Interacts (active GTP-bound form) with RASSF7 (PubMed:21278800). Interacts (active GTP-bound form) with both SHOC2 and PP1c (all isoforms) to form a tertiary complex; SHOC2 and PP1c preferably bind M-Ras/MRAS, but they also bind K-Ras/KRAS, N-Ras/NRAS and H-Ras/HRAS (PubMed:36175670, PubMed:35768504, PubMed:35831509, PubMed:35830882). {ECO:0000250\|UniProtKB:Q04970, ECO:0000269\|PubMed:21278800, ECO:0000269\|PubMed:35768504, ECO:0000269\|PubMed:35830882, ECO:0000269\|PubMed:35831509, ECO:0000269\|PubMed:36175670}.
Post-translational modification PTM: Palmitoylated by the ZDHHC9-GOLGA7 complex (PubMed:16000296). Depalmitoylated by ABHD17A, ABHD17B and ABHD17C (PubMed:26701913). A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (PubMed:15705808, PubMed:16000296, PubMed:2661017, PubMed:26701913). {ECO:0000269\|PubMed:15705808, ECO:0000269\|PubMed:16000296, ECO:0000269\|PubMed:2661017, ECO:0000269\|PubMed:26701913}.; PTM: Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). {ECO:0000250\|UniProtKB:P01116}.; PTM: Fatty-acylated at Lys-169 and/or Lys-170. {ECO:0000269\|PubMed:29239724}.; PTM: Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. {ECO:0000305\|PubMed:30442762}.; PTM: Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors. {ECO:0000269\|PubMed:30712867}.; PTM: (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. {ECO:0000269\|PubMed:19744486}.
Target Relevance information above includes information from UniProt accession: P01111
The UniProt Consortium

Data

Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 µM as determined in SPR assay (Biacore T200).

The purity of Mouse TIGIT is greater than 95% as determined by SEC-HPLC.

Mouse TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.