Mouse Siglec-5/CD170 Protein 3457

$315.00 – $1,050.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Thr17-Leu439

SKU: 3457parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q920G3
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1.
molecular weight	The protein has a predicted MW of 46.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Mouse Siglec-5/CD170 Protein 3457

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1.
Protein names Sialic acid-binding Ig-like lectin 5 (Siglec-5) (Sialic acid-binding Ig-like lectin F) (Siglec-F) (mSiglec-F) (CD antigen CD170)
Gene names Siglec5,Siglec5 Siglecf
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 10090Da
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Catalytic activity BINDING 114; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000250\|UniProtKB:O15389"; BINDING 120; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000250\|UniProtKB:O15389"; BINDING 122; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000250\|UniProtKB:O15389"
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Predominantly expressed by immature monocytic/myeloid lineage cells in bone marrow. Also found at lower levels in mature neutrophils and monocytes.
Domain Co
Target Relevance information above includes information from UniProt accession: Q920G3
The UniProt Consortium

Data

The purity of Mouse Siglec-5/CD170 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse Siglec-5/CD170 Protein

Mouse Siglec-5/CD170 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.