Mouse P-Selectin/CD62P Protein 3559

$210.00 – $700.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Trp42-Ala709

SKU: 3559parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q01102
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	P-selectin is critical in the progression of atherosclerosis as evidenced by knockout animal models where P-selectin knockout mice crossed with apoE deficient mice exhibit significantly reduced atherosclerosis and leukocyte recruitment in the plaque. A soluble form of P-selectin also exists, which may have pro-atherogenic and pro-thrombotic effects. Thus targeting of P-selectin remains a strong clinical candidate for developing novel therapeutic strategies in inflammatory diseases.
molecular weight	The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse P-Selectin/CD62P Protein 3559

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
P-selectin is critical in the progression of atherosclerosis as evidenced by knockout animal models where P-selectin knockout mice crossed with apoE deficient mice exhibit significantly reduced atherosclerosis and leukocyte recruitment in the plaque. A soluble form of P-selectin also exists, which may have pro-atherogenic and pro-thrombotic effects. Thus targeting of P-selectin remains a strong clinical candidate for developing novel therapeutic strategies in inflammatory diseases.
Protein names P-selectin (CD62 antigen-like family member P) (Granule membrane protein 140) (GMP-140) (Leukocyte-endothelial cell adhesion molecule 3) (LECAM3) (Platelet activation dependent granule-external membrane protein) (PADGEM) (CD antigen CD62P)
Gene names Selp,Selp Grmp
Protein family Selectin/LECAM family
Mass 10090Da
Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3) (By similarity).
Catalytic activity BINDING 121; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P16109"; BINDING 123; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000250\|UniProtKB:P16109"; BINDING 123; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P16109"; BINDING 124; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P16109"; BINDING 133; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000250\|UniProtKB:P16109"; BINDING 146; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000250\|UniProtKB:P16109"; BINDING 146; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P16109"; BINDING 147; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P16109"
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Structure Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. Interacts (via C-type lectin domain) with alpha-IIb/beta3 integrin ITGA2B:ITGB3 and alpha-V/beta-3 integrin ITGAV:ITGB3 (By similarity). Interacts with alpha5/beta1 integrin ITGA5:ITGB1 and alpha4/beta1 integrin ITGA4:ITGB (By similarity).
Domain Th
Target Relevance information above includes information from UniProt accession: Q01102
The UniProt Consortium

Data

The purity of Mouse P-Selectin/CD62P is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse P-Selectin/CD62P Protein

Mouse P-Selectin/CD62P on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.