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Mouse Noggin Protein 2401

$270.00$900.00

Summary

  • Expression: HEK293
  • Active: Yes (cell based assay)
  • Amino Acid Range: Gln28-Cys232
SKU: 2401parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P97466

express system

HEK293

product tag

C-hFc

purity

> 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC

background

Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.

molecular weight

The protein has a predicted MW of 49.83 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Mouse Noggin Protein 2401

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β-catenin, EGFR, ERK and Akt.
Protein names
Noggin
Gene names
Nog,Nog
Protein family
Noggin family
Mass
10090Da
Function
Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite (PubMed:9585504, PubMed:9603738). Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (By similarity).
Subellular location
Secreted.
Tissues
Expressed in condensing cartilage and immature chondrocytes.
Structure
Homodimer. Interacts with GDF5; inhibits chondrocyte differentiation.
Target Relevance information above includes information from UniProt accession: P97466
The UniProt Consortium

Cell based assay with Mouse Noggin Protein
Measured by its ability to inhibit BMP-4-induced alkaline phosphataseproduction by ATDC5 mouse chondrogenic cells. The ED for this effect is 0.1 - 0.4 ug/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.
HPLC of Mouse Noggin Protein
The purity of Mouse Noggin is greater than 90% as determined by SEC-HPLC.
SDS-PAGE gel of Mouse Noggin Protein
Mouse Noggin on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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