ELISA with Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Prot 3256

SDS-PAGE gel of Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer 3256

Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein 3256

$330.00 – $1,100.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe31-Val988(ITGAV)acidic tail & Gly22-Ser679(ITGB8)basic tail

SKU: 3256parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P43406(ITGAV)&Q0VBD0(ITGB8)
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation.
molecular weight	The protein has a predicted MW of 111.6(ITGAV)&76.6(ITGB8) kDa. Due to glycosylation, the protein migrates to 80-140 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein 3256

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation.
Protein names Integrin alpha-V (Vitronectin receptor subunit alpha) (CD antigen CD51) [Cleaved into: Integrin alpha-V heavy chain; Integrin alpha-V light chain]
Gene names Itgav,Itgav
Protein family Integrin alpha chain family
Mass 115360Da
Function The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, TGFB1 and vWF (PubMed:10025398, PubMed:9827803). They recognize the sequence R-G-D in a wide array of ligands. Alpha-V integrins may play a role in embryo implantation, angiogenesis and wound healing (PubMed:9827803). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (By similarity). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (By similarity). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (By similarity). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (By similarity). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (By similarity). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (By similarity). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (By similarity). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (By similarity). Integrin alpha-V/beta-6 or alpha-V/beta-8 (ITGAV:ITGB6 or ITGAV:ITGB8) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed:10025398, PubMed:25127859). ITGAV:ITGB3 acts as a receptor for CD40LG (By similarity). ITGAV:ITGB3 binds to the Lilrb4a/Gp49b receptor and enhances the Lilrb4a-mediated inhibition of mast cell activation (PubMed:11323698). ITGAV:ITGB3 also suppresses marginal zone B cell antibody production through its interaction with Lilrb4a (PubMed:24935931). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (By similarity).
Subellular location Cell membrane; Single-pass type I membrane protein. Cell junction, focal adhesion .
Structure Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-V (ITGAV) associates with either beta-1 (ITGB1), beta-3 (ITGB3), beta-5 (ITGB5), beta-6 (ITGB6) or beta-8 (ITGB8) (Probable). Interacts with RAB25. Interacts with CIB1 (By similarity). Integrins ITGAV:ITGB3 and ITGAV:ITGB5 interact with FBLN5 (via N-terminus) (PubMed:11805835). ITGAV:ITGB3 and ITGAV:ITGB5 interact with CCN3 (By similarity). ITGAV:ITGB3 interacts with ADGRA2 (By similarity). ITGAV:ITGB3 interacts with FGF2; it is likely that FGF2 can simultaneously bind ITGAV:ITGB3 and FGF receptors (By similarity). ITGAV:ITGB3 interacts with SELP (via C-type lectin domain); the interaction mediates cell-cell interaction and adhesion (By similarity). ITGAV:ITGB3 is found in a ternary complex with CX3CR1 and CX3CL1. ITGAV:ITGB3 is found in a ternary complex with NRG1 and ERBB3. ITGAV:ITGB3 is found in a ternary complex with FGF1 and FGFR1. ITGAV:ITGB3 is found in a ternary complex with IGF1 and IGF1R (By similarity). ITGAV:ITGB3 interacts with IGF2 (By similarity). ITGAV:ITGB3 and ITGAV:ITGB6 interact with FBN1 (By similarity). ITGAV:ITGB3 interacts with CD9, CD81 and CD151 (via second extracellular domain) (By similarity). ITGAV:ITGB6 interacts with TGFB1 (PubMed:10025398). ITGAV:ITGB3 interacts with PTN. Forms a complex with PTPRZ1 and PTN that stimulates endothelial cell migration through ITGB3 'Tyr-773' phosphorylation (By similarity).
Target Relevance information above includes information from UniProt accession: P43406
The UniProt Consortium

ELISA with Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Prot

Immobilized Mouse ITGAV&ITGB8, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.97µg/ml determined by ELISA.

HPLC of Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein

The purity of Mouse ITGAV&ITGB8 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer

Mouse ITGAV&ITGB8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. What is the specific application of the Mouse Integrin alpha V beta 8 (ITGAV/ITGB8) Heterodimer Protein 3256?
- The Mouse Integrin alpha V beta 8 Heterodimer Protein 3256 is commonly used in cell adhesion studies, integrin signaling pathway research, and investigating cell migration and differentiation processes.

2. Can this heterodimer protein be used in both in vitro and in vivo experiments?
- Yes, this Mouse Integrin alpha V beta 8 Heterodimer Protein 3256 is suitable for both in vitro and in vivo studies, making it versatile for a wide range of experimental settings.

3. How should I store this protein for optimal usage?
- It is recommended to store the Mouse Integrin alpha V beta 8 Heterodimer Protein 3256 at -20°C upon arrival. Avoid repeated freeze-thaw cycles to maintain its stability and functionality.

4. Is this mouse integrin protein compatible with various experimental techniques?
- Yes, the Mouse Integrin alpha V beta 8 Heterodimer Protein 3256 is compatible with applications such as Western blotting, ELISA, immunoprecipitation, flow cytometry, and cell adhesion assays, facilitating diverse research needs.

5. Can this heterodimer protein be used in conjunction with other antibodies or proteins in experiments?
- Absolutely, researchers often use this Mouse Integrin alpha V beta 8 Heterodimer Protein 3256 in combination with other antibodies or proteins to explore complex biological processes, such as cell motility and extracellular matrix interactions.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.