SPR with Mouse IFN alpha/beta R1 Protein 3598

Mouse IFN alpha/beta R1 Protein 3598

$300.00 – $1,000.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Glu27-Thr429

SKU: 3598parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P33896
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.
molecular weight	The protein has a predicted MW of 72.5 kDa. Due to glycosylation, the protein migrates to 83-110 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse IFN alpha/beta R1 Protein 3598

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.
Protein names Interferon alpha/beta receptor 1 (IFN-R-1) (IFN-alpha/beta receptor 1) (Type I interferon receptor 1)
Gene names Ifnar1,Ifnar1 Ifar Ifnar
Protein family Type II cytokine receptor family
Mass 10090Da
Function Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa) (PubMed:14532120, PubMed:1533935, PubMed:24075985). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves (By similarity). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes (By similarity). Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway (PubMed:23872679).
Subellular location Cell membrane ; Single-pass type I membrane protein. Late endosome. Lysosome. Note=Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes.
Structure Heterodimer with IFNAR2; forming the receptor for type I interferon (By similarity). Interacts with TYK2 (By similarity). Interacts with STAT1 and STAT2 (By similarity). Interacts (serine-phosphorylated form) with FBXW11, the substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (PubMed:14532120). 3Interacts with SHMT2; this promotes interaction with ABRAXAS2 and the BRISC complex (By similarity). Interacts with TRIM10; this interaction prevents association between IFNAR1 and TYK2 (By similarity).
Post-translational modification Ubiquitinated (PubMed:14532120). This leads to its internalization and lysosomal degradation. The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex; this prevents receptor internalization and degradation. Probable ubiquitination sites have been identified in human, but are poorly conserved across species.; Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120).
Target Relevance information above includes information from UniProt accession: P33896
The UniProt Consortium

Data

SPR with Mouse IFN alpha/beta R1 Protein

Mouse IFN alpha/beta R1, hFc Tag immobilized on CM5 Chip can bind Human IFN alpha 1, hFc Tag with an affinity constant of 20.35 nM as determined in SPR assay (Biacore T200).

The purity of Mouse IFNAR1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse IFN alpha/beta R1 Protein

Mouse IFNAR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.