SPR with Mouse GM-CSF R alpha Protein 4145

ELISA with Mouse GM-CSF R alpha Protein 4145

Mouse GM-CSF R alpha Protein 4145

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Leu30-Pro327

SKU: 4145parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q00941
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
molecular weight	The protein has a predicted MW of 33.1 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse GM-CSF R alpha Protein 4145

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
Protein names Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF-R-alpha) (GMCSFR-alpha) (GMR-alpha) (CD antigen CD116)
Gene names Csf2ra,Csf2ra Csfgmra
Protein family Type I cytokine receptor family, Type 5 subfamily
Mass 10090Da
Function Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
Subellular location Membrane ; Single-pass type I membrane protein .
Structure Heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits (By similarity).
Domain Th
Target Relevance information above includes information from UniProt accession: Q00941
The UniProt Consortium

Data

Mouse GM-CSF R alpha, His Tag captured on CM5 Chip via Anti-his antibody can bind Mouse GM-CSF, No Tag with an affinity constant of 6.32 nM as determined in SPR assay (Biacore T200).

Immobilized Mouse GM-CSF R alpha, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA (QC Test).

The purity of Mouse GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse GM-CSF R alpha Protein

Mouse GM-CSF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.