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Mouse GFRAL/GFR alpha-like Protein 4713

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln20-Glu350
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q6SJE0

express system

HEK293

product tag

N-His-Avi

purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

molecular weight

The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1 EU per μg by the LAL method.

Mouse GFRAL/GFR alpha-like Protein 4713

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Protein names
GDNF family receptor alpha-like
Gene names
Gfral,Gfral Gral
Protein family
GDNFR family
Mass
10090Da
Function
FUNCTION: Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomitting, and/or loss of appetite in response to various stresses (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:31152154, PubMed:31928886, PubMed:32723474, PubMed:33593916, PubMed:35202387, PubMed:36630958, PubMed:37380764). The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:31928886, PubMed:32723474, PubMed:33593916, PubMed:35202387, PubMed:36630958). The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin (PubMed:31875646, PubMed:31928886, PubMed:32661391, PubMed:32723474). Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways (PubMed:28846098, PubMed:28846099, PubMed:32723474, PubMed:33593916). Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed:28846098, PubMed:28846099, PubMed:31152154, PubMed:32723474). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (PubMed:32661391). {ECO:0000269|PubMed:28846097, ECO:0000269|PubMed:28846098, ECO:0000269|PubMed:28846099, ECO:0000269|PubMed:28953886, ECO:0000269|PubMed:31152154, ECO:0000269|PubMed:31875646, ECO:0000269|PubMed:31928886, ECO:0000269|PubMed:32661391, ECO:0000269|PubMed:32723474, ECO:0000269|PubMed:33593916, ECO:0000269|PubMed:35202387, ECO:0000269|PubMed:36630958, ECO:0000269|PubMed:37380764}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q6UXV0}; Single-pass membrane protein {ECO:0000305}; Extracellular side {ECO:0000305}.
Tissues
TISSUE SPECIFICITY: Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius. {ECO:0000269|PubMed:28846097, ECO:0000269|PubMed:28846099, ECO:0000269|PubMed:28953886}.
Structure
SUBUNIT: Interacts (via the extracellular domain) with GDF15 and RET; receptor of GDF15, mediates cellular signaling through interaction with RET after GDF15-binding (PubMed:28846098, PubMed:28846099). Interaction with RET requires previous GDF15-binding (PubMed:28846099). {ECO:0000269|PubMed:28846098, ECO:0000269|PubMed:28846099}.
Post-translational modification
PTM: Cleaved and inactivated by MMP14, inhibiting the GDF15-GFRAL aversive response. {ECO:0000305|PubMed:35177851}.
Target Relevance information above includes information from UniProt accession : Q6SJE0
The UniProt Consortium

Data

SPR with Mouse GFRAL/GFR alpha-like Protein
Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
ELISA with Mouse GFRAL/GFR alpha-like Protein
Immobilized Mouse GFRAL, His Tag at 2 µg/ml (100 µl/Well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test).
HPLC of Mouse GFRAL/GFR alpha-like Protein
The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Mouse GFRAL/GFR alpha-like Protein
Mouse GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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