Mouse FOLR4/Juno Protein 3562

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Gly20-Gly222

SKU: 3562parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9EQF4
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.
molecular weight	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse FOLR4/Juno Protein 3562

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.
Protein names Sperm-egg fusion protein Juno (Folate receptor 4) (Folate receptor delta) (FR-delta) (Folate-binding protein 3) (IZUMO1 receptor protein JUNO)
Gene names Izumo1r,Izumo1r Folbp3 Folr4 Juno
Protein family Folate receptor family
Mass 10090Da
Function Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization (PubMed:24739963, PubMed:26859261, PubMed:27309808, PubMed:27416963). The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion (PubMed:24739963, PubMed:26859261, PubMed:27309808). The ligand-receptor interaction probably does not act as a membrane 'fusogen' (PubMed:24739963, PubMed:26859261, PubMed:27309808). Does not bind folate (PubMed:24739963).
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor. Note=GPI-anchored at the oolemma.
Tissues Widely expressed with higher expression in thymus, spleen and lung (PubMed:11111049). Present at the cell surface of unfertilized oocytes, while it is barely detectable 30 to 40 minutes after fertilization (at protein level) (PubMed:24739963).
Structure Monomer (PubMed:26859261). Interacts with IZUMO1; the interaction is direct (PubMed:24739963, PubMed:25209248, PubMed:26859261, PubMed:27309808, PubMed:27416963, PubMed:32484434). IZUMO1 and IZUMO1R/JUNO form a complex with 1:1 stoichiometry (By similarity).
Post-translational modification The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage (PubMed:24739963). Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm (PubMed:24739963).
Target Relevance information above includes information from UniProt accession: Q9EQF4
The UniProt Consortium

Data

The purity of Mouse FOLR4 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse FOLR4/Juno Protein

Mouse FOLR4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.