ELISA with Mouse Fas/TNFRSF6/CD95 Protein 5116

Mouse Fas/TNFRSF6/CD95 Protein 5116

$180.00 – $600.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln22-Arg169

SKU: 5116parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P25446
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).
molecular weight	The protein has a predicted MW of 43.3 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse Fas/TNFRSF6/CD95 Protein 5116

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).
Protein names Tumor necrosis factor receptor superfamily member 6 (Apo-1 antigen) (Apoptosis-mediating surface antigen FAS) (FASLG receptor) (CD antigen CD95)
Gene names Fas,Fas Apt1 Tnfrsf6
Mass 10090Da
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane raft .
Tissues Detected in various tissues including thymus, liver, lung, heart, and adult ovary.
Structure Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits (By similarity). Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (PubMed:15383280). Binds DAXX (PubMed:9215629). Interacts with HIPK3 (PubMed:11034606). Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BABAM2 and FEM1B. Interacts with CALM (By similarity). In the absence of stimulation, interacts with BIRC2, DDX3X and GSK3B. The interaction with BIRC2 and DDX3X is further enhanced upon receptor stimulation and accompanied by DDX3X and BIRC2 cleavage (By similarity).
Post-translational modification Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane.
Domain Co
Target Relevance information above includes information from UniProt accession: P25446
The UniProt Consortium

Data

ELISA with Mouse Fas/TNFRSF6/CD95 Protein

Immobilized Human Fas Ligand, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Mouse Fas, hFc Tag with the EC50 of 0.25µg/ml determined by ELISA.

The purity of Mouse Fas is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse Fas/TNFRSF6/CD95 Protein

Mouse Fas on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Frequently Asked Questions

1. What is the molecular weight of the Mouse Fas (TNFRSF6/CD95) Protein (5116)?
- The molecular weight of the Mouse Fas (TNFRSF6/CD95) Protein (5116) is approximately 43.7 kDa.

2. How should I store the Mouse Fas (TNFRSF6/CD95) Protein (5116) for optimal preservation?
- It is recommended to store the Mouse Fas (TNFRSF6/CD95) Protein (5116) at -20°C upon arrival. Avoid multiple freeze-thaw cycles to maintain protein stability.

3. Is the Mouse Fas (TNFRSF6/CD95) Protein (5116) suitable for use in flow cytometry applications?
- Yes, the Mouse Fas (TNFRSF6/CD95) Protein (5116) is compatible with flow cytometry applications, making it a versatile tool for studying Fas-mediated signaling pathways and cellular interactions.

4. Can the Mouse Fas (TNFRSF6/CD95) Protein (5116) be used for in vitro experiments?
- Yes, the Mouse Fas (TNFRSF6/CD95) Protein (5116) can be used for in vitro experiments such as cell culture studies, protein-protein interaction assays, and functional studies related to apoptosis and cell death mechanisms.

5. Is the Mouse Fas (TNFRSF6/CD95) Protein (5116) recommended for use in combination with other antibodies or proteins?
- Yes, the Mouse Fas (TNFRSF6/CD95) Protein (5116) can be used in combination with other antibodies or proteins to investigate complex signaling pathways, protein-protein interactions, and downstream effects of Fas activation in various biological systems.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.