Mouse DSG3 Protein 5130

$270.00 – $900.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Glu50-Pro617

SKU: 5130parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O35902
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	To identify genes that could potentially serve as molecular therapeutic markers for human head and neck cancer (HNC), DSG3 is identified overexpressed in HNC, with the degree of overexpression associated with clinicopathologic features of the tumor. Inhibition of DSG3 significantly suppresses carcinogenic potential in cellular and in vivo animal studies. DSG3 is a potential molecular target in the development of adjuvant therapy for HNC.
molecular weight	The protein has a predicted MW of 64.3 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse DSG3 Protein 5130

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
To identify genes that could potentially serve as molecular therapeutic markers for human head and neck cancer (HNC),DSG3 is identified overexpressed in HNC, with the degree of overexpression associated with clinicopathologic features of the tumor. Inhibition of DSG3 significantly suppresses carcinogenic potential in cellular and in vivo animal studies. DSG3 is a potential molecular target in the development of adjuvant therapy for HNC.
Protein names Desmoglein-3 (130 kDa pemphigus vulgaris antigen homolog)
Gene names Dsg3,Dsg3
Mass 107889Da
Function FUNCTION: A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed:9166409). Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes (By similarity). Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle (PubMed:9166409, PubMed:9701552). Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (PubMed:9166409). May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3 (By similarity). Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG (By similarity). May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation (By similarity). Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1 (By similarity). The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity (By similarity). Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing (PubMed:26763450). {ECO:0000250\|UniProtKB:P32926, ECO:0000250\|UniProtKB:Q7YRU7, ECO:0000269\|PubMed:26763450, ECO:0000269\|PubMed:9166409, ECO:0000269\|PubMed:9701552}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:9701552}; Single-pass type I membrane protein {ECO:0000255}. Cell junction, desmosome {ECO:0000269\|PubMed:9166409}. Cytoplasm {ECO:0000250\|UniProtKB:P32926}. Cell junction, tight junction {ECO:0000250\|UniProtKB:Q7YRU7}. Cell junction {ECO:0000269\|PubMed:18343367}.
Tissues TISSUE SPECIFICITY: Expressed in the basal layer of the outer root sheath of the telogen hair club, specifically at the cell membrane between the apex of the cells and the surrounding hair club (at protein level) (PubMed:9701552). Expression is less abundant between the lateral margins of the outer root sheath basal cells (at protein level) (PubMed:9701552). Expressed in epidermis (PubMed:12787123). Expressed in the epithelium of the tongue (PubMed:9166409). {ECO:0000269\|PubMed:12787123, ECO:0000269\|PubMed:9166409, ECO:0000269\|PubMed:9701552}.
Structure SUBUNIT: Homodimer (PubMed:30949721). Part of a complex that contains DSG3, PKP1, YAP1 and YWHAG; the complex is required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes (By similarity). Interacts with PKP2. Interacts with CTNND1; the interaction facilitates DSG3 localization and retention at cell-cell junctions (PubMed:18343367). Interacts with CDH1; the interaction is required for CDH1 localization to developing adherens junctions (By similarity). Interacts with RAC1; the interaction is required for DSG3 translocation to cell-cell junctions, organization of cortical F-actin bundles and actin anchoring at cell-cell junctions (By similarity). Interacts with DSC3; the interaction may limit the interaction of DSC3 with p38MAPK family members and therefore repress p38MAPK signaling activation (PubMed:26763450). {ECO:0000250\|UniProtKB:P32926, ECO:0000269\|PubMed:18343367, ECO:0000269\|PubMed:26763450, ECO:0000269\|PubMed:30949721}.
Domain DOMAIN: Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. {ECO:0000250}.
Target Relevance information above includes information from UniProt accession: O35902
The UniProt Consortium

Data

The purity of Mouse DSG3 is greater than 95% as determined by SEC-HPLC.

Mouse DSG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.