Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
accession | O55186 |
express system | HEK293 |
product tag | C-hFc |
purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
background | a CD59a fusion protein bound specifically to mouse B cells, and enhanced B cell proliferation in a MLR, demonstrating that B cells express an as yet unidentified ligand for CD59a that aids in B cell activation. |
molecular weight | The protein has a predicted MW of 35.17 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result. |
available size | 100 µg, 500 µg |
endotoxin | Less than 1EU per μg by the LAL method. |
Mouse CD59a Protein 3292
$270.00 – $900.00
Summary
- Expression: HEK293
- Pure: Yes (HPLC)
- Amino Acid Range: Leu24-Ser96
Mouse CD59a Protein 3292
protein |
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Size and concentration 100, 500µg and liquid |
Form Liquid |
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Storage buffer Shipped with dry ice. |
Purity > 95% as determined by Tris-Bis PAGE |
target relevance |
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a CD59a fusion protein bound specifically to mouse B cells, and enhanced B cell proliferation in a MLR, demonstrating that B cells express an as yet unidentified ligand for CD59a that aids in B cell activation. |
Protein names CD59A glycoprotein (MAC-inhibitory protein) (MAC-IP) (Membrane attack complex inhibition factor) (MACIF) (Protectin) (CD antigen CD59) |
Gene names Cd59a,Cd59a Cd59 |
Mass 10090Da |
Function Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (By similarity). |
Subellular location Cell membrane; Lipid-anchor, GPI-anchor. |
Tissues Expressed in all tissues examined (liver, kidney, spleen, thymus, brain and heart). Low levels in thymus. Also expressed in mononuclear cells, erythrocytes and platelets. Barely detected in neutrophils. |
Target Relevance information above includes information from UniProt accession: O55186 |
The UniProt Consortium |
The purity of Mouse CD59a is greater than 95% as determined by SEC-HPLC. |
Mouse CD59a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
1. Question: What is the protein concentration of the Mouse CD59a Protein (3292) product?
Answer: The protein concentration of Mouse CD59a Protein (3292) is 0.1 mg/ml.
2. Question: How should I store the Mouse CD59a Protein (3292) after receiving it?
Answer: The Mouse CD59a Protein (3292) should be stored at -80°C upon arrival to ensure long-term stability and activity.
3. Question: Is the Mouse CD59a Protein (3292) compatible with specific applications?
Answer: Yes, the Mouse CD59a Protein (3292) is suitable for various applications, including western blotting, ELISA, immunoprecipitation, and functional assays.
4. Question: Can this Mouse CD59a Protein (3292) be used for cell culture experiments?
Answer: Yes, the Mouse CD59a Protein (3292) can be used for cell culture experiments after reconstitution in the appropriate buffer.
5. Question: Are there any specific handling instructions for using the Mouse CD59a Protein (3292)?
Answer: It is recommended to avoid repeated freeze-thaw cycles and to aliquot the protein upon receiving to avoid multiple freeze-thaw cycles.
Answer: The protein concentration of Mouse CD59a Protein (3292) is 0.1 mg/ml.
2. Question: How should I store the Mouse CD59a Protein (3292) after receiving it?
Answer: The Mouse CD59a Protein (3292) should be stored at -80°C upon arrival to ensure long-term stability and activity.
3. Question: Is the Mouse CD59a Protein (3292) compatible with specific applications?
Answer: Yes, the Mouse CD59a Protein (3292) is suitable for various applications, including western blotting, ELISA, immunoprecipitation, and functional assays.
4. Question: Can this Mouse CD59a Protein (3292) be used for cell culture experiments?
Answer: Yes, the Mouse CD59a Protein (3292) can be used for cell culture experiments after reconstitution in the appropriate buffer.
5. Question: Are there any specific handling instructions for using the Mouse CD59a Protein (3292)?
Answer: It is recommended to avoid repeated freeze-thaw cycles and to aliquot the protein upon receiving to avoid multiple freeze-thaw cycles.
Publications
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