Mouse CD47 Protein 3297

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln19-Lys140

SKU: 3297parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q61735
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
molecular weight	The protein has a predicted MW of 40.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse CD47 Protein 3297

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Protein names Leukocyte surface antigen CD47 (Integrin-associated protein) (IAP) (CD antigen CD47)
Gene names Cd47,Cd47
Mass 10090Da
Function Adhesive protein that mediates cell-to-cell interactions (By similarity). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (By similarity). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:20610415, PubMed:23591719, PubMed:27742621). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (PubMed:20610415). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (By similarity). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (PubMed:15917238). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (PubMed:18156939). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (PubMed:23591719). May play a role in membrane transport and/or integrin dependent signal transduction (By similarity). May prevent premature elimination of red blood cells (PubMed:10856220).
Subellular location Cell membrane ; Multi-pass membrane protein .
Structure Monomer (By similarity). Interacts with THBS1 (via the C-terminal domain) (By similarity). Interacts with SIRPA (PubMed:10856220). Interacts with FAS/CD95; interaction may be enhanced by functional activation (By similarity). Interacts with SIRPG, UBQLN1 and UBQLN2 (By similarity). May interact with fibrinogen (By similarity).
Target Relevance information above includes information from UniProt accession: Q61735
The UniProt Consortium

Mouse CD47, hFc Tag captured on CM5 Chip via Protein A can bind Mouse SIRP alpha, His Tag with an affinity constant of 28.33 nM as determined in SPR assay (Biacore T200).

Immobilized Mouse SIRP alpha, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26µg/ml determined by ELISA (QC Test).

The purity of Mouse CD47 is greater than 95% as determined by SEC-HPLC.

Mouse CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Frequently Asked Questions

1. What is the molecular weight of the Mouse CD47 Protein (3297) offered on your website?
- The molecular weight of the Mouse CD47 Protein (3297) is approximately 32 kDa.

2. How should I store the Mouse CD47 Protein (3297) for optimal preservation?
- The Mouse CD47 Protein (3297) should be stored at -20°C upon arrival and can be kept at -80°C for long-term storage. Avoid repeated freeze-thaw cycles.

3. Is the Mouse CD47 Protein (3297) suitable for use in flow cytometry applications?
- Yes, the Mouse CD47 Protein (3297) can be used in flow cytometry applications. Please refer to the product datasheet for specific recommended dilutions and protocols.

4. Can I use the Mouse CD47 Protein (3297) for Western blotting experiments?
- Yes, the Mouse CD47 Protein (3297) is suitable for Western blotting applications. Follow standard protocols for Western blot analysis with the recommended dilutions provided in the product datasheet.

5. Is the Mouse CD47 Protein (3297) compatible with my specific research model or system?
- The Mouse CD47 Protein (3297) has been validated for use in various research models and systems, but we recommend consulting the literature or contacting our technical support team for further information on compatibility with your specific research needs.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.