Mouse CD19 Protein 3479

$375.00 – $1,250.00

Summary

Expression: HEK293
Pure: Yes (SDS-PAGE)
Amino Acid Range: Arg19-Gly287

SKU: 3479parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P25918
express system	HEK293
product tag	C-His
purity	> 90% as determined by Tris-Bis PAGE
background	CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
molecular weight	The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Mouse CD19 Protein 3479

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
Protein names B-lymphocyte antigen CD19 (Differentiation antigen CD19) (CD antigen CD19)
Gene names Cd19,Cd19
Mass 10090Da
Function Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:20101619, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:7542548, PubMed:7543183, PubMed:9317126). Required for normal differentiation of B-1 cells (PubMed:12387743, PubMed:7542548, PubMed:7543183). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:12387743, PubMed:7542548, PubMed:9317126). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:7542548, PubMed:7543183).
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane raft ; Single-pass type I membrane protein .
Tissues Detected on B cells in spleen, bone marrow, thymus and lymph nodes (PubMed:12387743, PubMed:20101619, PubMed:7542548). Detected on peripheral blood lymphocytes (at protein level) (PubMed:7543183).
Structure Interacts with CR2/CD21. Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells. Interacts directly with CD81 (via the second extracellular domain); this interaction is initiated early during biosynthesis in the ER/pre-Golgi compartments and is essential for trafficking and compartmentalization of CD19 receptor on the cell surface of when phosphorylated on Tyr-346 and/or Tyr-376. Interacts with PLCG2 when phosphorylated on Tyr-402. Interacts with LYN. Interacts (when tyrosine phosphorylated) with the regulatory p85 subunit of phosphatidylinositol 3-kinase (PIK3R1 or PIK3R2).
Post-translational modification Phosphorylated on tyrosine following B-cell activation (PubMed:20101619). Phosphorylated on tyrosine residues by LYN (By similarity). Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-522 is extremely rapid, followed by phosphorylation at Tyr-402. In contrast, phosphorylation of Tyr-493 appears more slowly and is more transient, returning rapidly to basal levels (PubMed:20101619).
Target Relevance information above includes information from UniProt accession: P25918
The UniProt Consortium

Mouse CD19 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

1. **Question:** What is the concentration of the Mouse CD19 Protein (3479)?
**Answer:** The concentration of the Mouse CD19 Protein (3479) is 0.1 mg/mL.

2. **Question:** How should I store the Mouse CD19 Protein (3479) for optimal stability?
**Answer:** The Mouse CD19 Protein (3479) should be stored at -20°C upon arrival. For long-term storage, it is recommended to store it at -80°C.

3. **Question:** Is the Mouse CD19 Protein (3479) compatible with flow cytometry applications?
**Answer:** Yes, the Mouse CD19 Protein (3479) is suitable for flow cytometry applications. It can be used for various immunological assays, including flow cytometry and western blotting.

4. **Question:** What is the purity level of the Mouse CD19 Protein (3479)?
**Answer:** The Mouse CD19 Protein (3479) has a purity level of greater than 90%, ensuring high quality and reliable performance in experiments.

5. **Question:** Can the Mouse CD19 Protein (3479) be used for in vitro research?
**Answer:** Yes, the Mouse CD19 Protein (3479) is intended for in vitro research purposes only. It is not for therapeutic or diagnostic use.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.