SPR with Mouse B7-H2/ICOSLG Protein 4257

Mouse B7-H2/ICOSLG Protein 4257

$270.00 – $900.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Glu47-Lys279

SKU: 4257parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9JHJ8
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
molecular weight	The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse B7-H2/ICOSLG Protein 4257

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Protein names ICOS ligand (B7 homolog 2) (B7-H2) (B7-like protein Gl50) (B7-related protein 1) (B7RP-1) (LICOS) (CD antigen CD275)
Gene names Icoslg,Icoslg B7h2 B7rp1 Icosl
Protein family Immunoglobulin superfamily, BTN/MOG family
Mass 10090Da
Function Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Isoform 1 highest expression in lymphoid tissues, such as spleen (mostly in the marginal zone), lymph nodes (particularly in the cortex and in both primary and secondary follicles), thymus (predominantly in the medulla) and Peyer patches (mostly in the follicles), lower levels in many non-lymphoid tissues, such as brain, heart, kidney, liver, lung, skeletal muscle and testis. Present on freshly isolated splenic B-cells, T-cells, dendritic cells and macrophages. The expression of isoform 2 is restricted to heart, spleen and kidney.
Structure Interacts with CTLA4 (in vitro).
Target Relevance information above includes information from UniProt accession: Q9JHJ8
The UniProt Consortium

Data

Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 µM as determined in SPR assay (Biacore T200).

The purity of Mouse B7-H2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse B7-H2/ICOSLG Protein

Mouse B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.