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Human TNFR2/CD120b/TNFRSF1B Protein 4669

$240.00$800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Leu23-Asp257
SKU: 4669parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P20333

express system

HEK293

product tag

C-mFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.

molecular weight

The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human TNFR2/CD120b/TNFRSF1B Protein 4669

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.
Protein names
Tumor necrosis factor receptor superfamily member 1B (Tumor necrosis factor receptor 2) (TNF-R2) (Tumor necrosis factor receptor type II) (TNF-RII) (TNFR-II) (p75) (p80 TNF-alpha receptor) (CD antigen CD120b) (Etanercept) [Cleaved into: Tumor necrosis factor receptor superfamily member 1b, membrane form; Tumor necrosis factor-binding prot
Gene names
TNFRSF1B,TNFRSF1B TNFBR TNFR2
Mass
9606Da
Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Subellular location
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Tumor necrosis factor-binding protein 2]: Secreted.
Structure
Binds to TRAF2 (PubMed:10206649, PubMed:8069916). Interacts with BMX (PubMed:12370298). Interacts (activated form) with XPNPEP3 (PubMed:25609706).
Post-translational modification
Phosphorylated; mainly on serine residues and with a very low level on threonine residues.; A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Target Relevance information above includes information from UniProt accession: P20333
The UniProt Consortium

Data

SPR with Human TNFR2/CD120b/TNFRSF1B Protein
Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
ELISA with Human TNFR2/CD120b/TNFRSF1B Protein
Immobilized Human TNF alpha, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).
ELISA with Human TNFR2/CD120b/TNFRSF1B Protein
Immobilized Human TNFR2, mFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.
HPLC of Human TNFR2/CD120b/TNFRSF1B Protein
The purity of Human TNFR2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human TNFR2/CD120b/TNFRSF1B Protein
Human TNFR2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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