Skip to content

Human TNFR2/CD120b/TNFRSF1B Protein 4517

$240.00$800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Leu23-Asp257
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P20333

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

molecular weight

The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human TNFR2/CD120b/TNFRSF1B Protein 4517

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Protein names
Tumor necrosis factor receptor superfamily member 1B (Tumor necrosis factor receptor 2) (TNF-R2) (Tumor necrosis factor receptor type II) (TNF-RII) (TNFR-II) (p75) (p80 TNF-alpha receptor) (CD antigen CD120b) (Etanercept) [Cleaved into: Tumor necrosis factor receptor superfamily member 1b, membrane form; Tumor necrosis factor-binding prot
Gene names
TNFRSF1B,TNFRSF1B TNFBR TNFR2
Mass
9606Da
Function
FUNCTION: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. {ECO:0000269|PubMed:12370298}.
Subellular location
SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted.; SUBCELLULAR LOCATION: [Tumor necrosis factor-binding protein 2]: Secreted.
Structure
SUBUNIT: Binds to TRAF2 (PubMed:10206649, PubMed:8069916). Interacts with BMX (PubMed:12370298). Interacts (activated form) with XPNPEP3 (PubMed:25609706). {ECO:0000269|PubMed:10206649, ECO:0000269|PubMed:12370298, ECO:0000269|PubMed:25609706, ECO:0000269|PubMed:8069916}.
Post-translational modification
PTM: Phosphorylated; mainly on serine residues and with a very low level on threonine residues.; PTM: A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
Target Relevance information above includes information from UniProt accession : P20333
The UniProt Consortium

Data

ELISA with Human TNFR2/CD120b/TNFRSF1B Protein
Immobilized Human TNFR2, His Tag at 5µg/ml (100µl/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test).
ELISA with Human TNFR2/CD120b/TNFRSF1B Protein
Immobilized Human TNFR2, His Tag at 0.5µg/ml (100µl/Well). Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
HPLC of Human TNFR2/CD120b/TNFRSF1B Protein
The purity of Human TNFR2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human TNFR2/CD120b/TNFRSF1B Protein
Human TNFR2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.