Human TIMP1 Protein 3251

$255.00 – $850.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Cys24-Ala207

SKU: 3251parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_003245
express system	HEK293
product tag	N-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Tissue inhibitor of metalloprotease-1 (TIMP-1) is a tissue inhibitor of matrix metalloproteinases (MMPs). It however exerts multiple effects on biological processes, such as cell growth, proliferation, differentiation and apoptosis, in an MMP-independent manner.
molecular weight	The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human TIMP1 Protein 3251

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Tissue inhibitor of metalloprotease-1 (TIMP-1) is a tissue inhibitor of matrix metalloproteinases (MMPs). It however exerts multiple effects on biological processes, such as cell growth, proliferation, differentiation and apoptosis, in an MMP-independent manner.
Protein names Metalloproteinase inhibitor 1 (Collagenase inhibitor 16C8 fibroblast) (Erythroid-potentiating activity) (EPA) (TPA-S1) (TPA-induced protein) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
Gene names Timp1,Timp1 Timp Timp-1
Protein family Protease inhibitor I35 (TIMP) family
Mass 10090Da
Function Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
Catalytic activity BINDING 25; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_note="ligand shared with metalloproteinase partner"; /evidence="ECO:0000250\|UniProtKB:P16035"
Subellular location Secreted .
Tissues Found in fetal and adult tissues. Highest levels are found in bone. Also found in lung, ovary and uterus.
Structure Interacts with MMP1, MMP3, MMP10 and MMP13, but has only very low affinity for MMP14 (By similarity). Interacts with CD63; identified in a complex with CD63 and ITGB1.
Post-translational modification The activity of TIMP1 is dependent on the presence of disulfide bonds.; N-glycosylated.
Target Relevance information above includes information from UniProt accession: P12032
The UniProt Consortium

Data

The purity of Human TIMP1 is greater than 95% as determined by SEC-HPLC.

Human TIMP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.