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Human TGF-beta RII/TGFBR2 Protein 4704

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ile24-Asp159
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P37173

express system

HEK293

product tag

N-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

The TGFBR2 gene provides instructions for making a protein called transforming growth factor-beta (TGF-β) receptor type 2. This receptor transmits signals from the cell surface into the cell through a process called signal transduction. Through this type of signaling, the environment outside the cell affects activities inside the cell such as stimulation of cell growth and division.

molecular weight

The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human TGF-beta RII/TGFBR2 Protein 4704

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The TGFBR2 gene provides instructions for making a protein called transforming growth factor-beta (TGF-β) receptor type 2. This receptor transmits signals from the cell surface into the cell through a process called signal transduction. Through this type of signaling, the environment outside the cell affects activities inside the cell such as stimulation of cell growth and division.
Protein names
TGF-beta receptor type-2 (TGFR-2) (EC 2.7.11.30) (TGF-beta type II receptor) (Transforming growth factor-beta receptor type II) (TGF-beta receptor type II) (TbetaR-II)
Gene names
TGFBR2,TGFBR2
Protein family
Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfam
Mass
9606Da
Function
FUNCTION: Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and thus regulates a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. {ECO:0000269|PubMed:7774578}.; FUNCTION: [Isoform 1]: Has transforming growth factor beta-activated receptor activity. {ECO:0000269|PubMed:8635485}.; FUNCTION: [Isoform 2]: Has transforming growth factor beta-activated receptor activity. {ECO:0000269|PubMed:8635485}.; FUNCTION: [Isoform 3]: Binds TGFB1, TGFB2 and TGFB3 in the picomolar affinity range without the participation of additional receptors. Blocks activation of SMAD2 and SMAD3 by TGFB1. {ECO:0000269|PubMed:34568316}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1310899, ECO:0000269|PubMed:25893292}; Single-pass type I membrane protein {ECO:0000269|PubMed:1310899}. Membrane raft {ECO:0000269|PubMed:25893292}.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted {ECO:0000269|PubMed:34568316}.
Structure
SUBUNIT: Homodimer. Heterohexamer; TGFB1, TGFB2 and TGFB3 homodimeric ligands assemble a functional receptor composed of two TGFBR1 and TGFBR2 heterodimers to form a ligand-receptor heterohexamer. The respective affinity of TGFRB1 and TGFRB2 for the ligands may modulate the kinetics of assembly of the receptor and may explain the different biological activities of TGFB1, TGFB2 and TGFB3. Component of a complex composed of TSC22D1 (via N-terminus), TGFBR1 and TGFBR2; the interaction between TSC22D1 and TGFBR1 is inhibited by SMAD7 and promoted by TGFB1 (PubMed:21791611). Interacts with DAXX. Interacts with DYNLT4. Interacts with ZFYVE9; ZFYVE9 recruits SMAD2 and SMAD3 to the TGF-beta receptor. Interacts with and is activated by SCUBE3; this interaction does not affect TGFB1-binding to TGFBR2. Interacts with VPS39; this interaction is independent of the receptor kinase activity and of the presence of TGF-beta. Interacts with CLU (PubMed:8555189). {ECO:0000269|PubMed:11483955, ECO:0000269|PubMed:11850637, ECO:0000269|PubMed:12941698, ECO:0000269|PubMed:16982625, ECO:0000269|PubMed:18243111, ECO:0000269|PubMed:20207738, ECO:0000269|PubMed:21441952, ECO:0000269|PubMed:21791611, ECO:0000269|PubMed:8555189, ECO:0000269|PubMed:9865696}.; SUBUNIT: [Isoform 3]: Homodimer; disulfide-linked. {ECO:0000269|PubMed:34568316}.
Post-translational modification
PTM: Phosphorylated on a Ser/Thr residue in the cytoplasmic domain.
Target Relevance information above includes information from UniProt accession : P37173
The UniProt Consortium

Data

SPR with Human TGF-beta RII/TGFBR2 Protein
Human TGF-beta RII, hFc Tag captured on CM5 Chip via Protein A can bind Human Latent TGF beta 2, His Tag with an affinity constant of 2.21 µM as determined in SPR assay (Biacore T200).
ELISA with Human TGF-beta RII/TGFBR2 Protein
Immobilized Human Mature TGF beta 1, No Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).
HPLC of Human TGF-beta RII/TGFBR2 Protein
The purity of Human TGF-beta RII is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human TGF-beta RII/TGFBR2 Protein
Human TGF-beta RII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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