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Human SIRP alpha V2/CD172a Protein 4853

$270.00$900.00

Summary

  • Expression: HEK293
  • Functional: Yes (binding assay)
  • Amino Acid Range: Glu31-Arg369
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

AAH26692

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

molecular weight

The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human SIRP alpha V2/CD172a Protein 4853

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

SPR with Human SIRP alpha V2/CD172a Protein
Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay (Biacore T200).
ELISA with Human SIRP alpha V2/CD172a Protein
Immobilized Human SIRP alpha V2, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test).
ELISA with Human SIRP alpha V2/CD172a Protein
Immobilized Human SIRP alpha V2, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Binding assay with Human SIRP alpha V2/CD172a Protein
Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml.
HPLC of Human SIRP alpha V2/CD172a Protein
The purity of Human SIRP alpha V2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human SIRP alpha V2/CD172a Protein
Human SIRP alpha V2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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