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Human Siglec-5/CD170 Protein 4573

$210.00$700.00

Summary

  • Expression: HEK293
  • Pure: Yes (HPLC)
  • Amino Acid Range: Glu17-Thr434
SKU: 4573parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

O15389

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1.

molecular weight

The protein has a predicted MW of 73.1 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Siglec-5/CD170 Protein 4573

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1.
Protein names
Sialic acid-binding Ig-like lectin 5 (Siglec-5) (CD33 antigen-like 2) (Obesity-binding protein 2) (OB-BP2) (OB-binding protein 2) (CD antigen CD170)
Gene names
SIGLEC5,SIGLEC5 CD33L2 OBBP2
Protein family
Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass
9606Da
Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Catalytic activity
BINDING 119; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000269|PubMed:18022638"; BINDING 127; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000269|PubMed:18022638"; BINDING 129; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000269|PubMed:18022638"
Subellular location
Membrane; Single-pass type I membrane protein.
Tissues
Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
Domain
Co
Target Relevance information above includes information from UniProt accession: O15389
The UniProt Consortium

Data

HPLC of Human Siglec-5/CD170 Protein
The purity of Human Siglec-5 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Siglec-5/CD170 Protein
Human Siglec-5 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

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