Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
accession | O15389 |
express system | HEK293 |
product tag | C-hFc |
purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
background | Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. |
molecular weight | The protein has a predicted MW of 73.1 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result. |
available size | 100 µg, 500 µg |
endotoxin | Less than 1EU per μg by the LAL method. |
Human Siglec-5/CD170 Protein 4573
$210.00 – $700.00
Summary
- Expression: HEK293
- Pure: Yes (HPLC)
- Amino Acid Range: Glu17-Thr434
Human Siglec-5/CD170 Protein 4573
protein |
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Size and concentration 100, 500µg and lyophilized |
Form Lyophilized |
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Storage buffer Shipped at ambient temperature. |
Purity > 95% as determined by Tris-Bis PAGE |
target relevance |
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Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. |
Protein names Sialic acid-binding Ig-like lectin 5 (Siglec-5) (CD33 antigen-like 2) (Obesity-binding protein 2) (OB-BP2) (OB-binding protein 2) (CD antigen CD170) |
Gene names SIGLEC5,SIGLEC5 CD33L2 OBBP2 |
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family |
Mass 9606Da |
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. |
Catalytic activity BINDING 119; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000269|PubMed:18022638"; BINDING 127; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000269|PubMed:18022638"; BINDING 129; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000269|PubMed:18022638" |
Subellular location Membrane; Single-pass type I membrane protein. |
Tissues Expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. |
Domain Co |
Target Relevance information above includes information from UniProt accession: O15389 |
The UniProt Consortium |
Data
The purity of Human Siglec-5 is greater than 95% as determined by SEC-HPLC. |
Human Siglec-5 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.pmid | title | authors | citation |
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Protocols
relevant to this product |
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Documents
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