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Human Semaphorin 4D/SEMA4D/CD100 Protein 4852

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Met22-Arg734
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q92854

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

molecular weight

The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Semaphorin 4D/SEMA4D/CD100 Protein 4852

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.
Protein names
Semaphorin-4D (A8) (BB18) (GR3) (CD antigen CD100)
Gene names
SEMA4D,SEMA4D C9orf164 CD100 SEMAJ
Protein family
Semaphorin family
Mass
9606Da
Function
FUNCTION: Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569). Promotes the migration of cerebellar granule cells (PubMed:16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703). {ECO:0000250|UniProtKB:O09126, ECO:0000269|PubMed:16055703, ECO:0000269|PubMed:19788569, ECO:0000269|PubMed:20877282, ECO:0000269|PubMed:8876214}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20877282, ECO:0000269|PubMed:8876214}; Single-pass type I membrane protein {ECO:0000255}.
Tissues
TISSUE SPECIFICITY: Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. {ECO:0000269|PubMed:8876214}.
Structure
SUBUNIT: Homodimer (PubMed:20877282). Interacts with PLXNB2 (By similarity). Interacts with PLXNB1 (PubMed:10520995). {ECO:0000250|UniProtKB:O09126, ECO:0000269|PubMed:10520995, ECO:0000269|PubMed:20877282}.
Target Relevance information above includes information from UniProt accession : Q92854
The UniProt Consortium

Data

ELISA with Human Semaphorin 4D/SEMA4D/CD100 Protein
Immobilized Human Semaphorin 4D, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
HPLC of Human Semaphorin 4D/SEMA4D/CD100 Protein
The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Semaphorin 4D/SEMA4D/CD100 Protein
Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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