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Human SCF Protein 2683

$203.00$675.00

Summary

  • Expression: E.coli
  • Active: Yes (cell based assay)
  • Amino Acid Range: Glu26-Ala189
SKU: 2683parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P21583

express system

E.coli

product tag

No Tag

purity

> 95% as determined by Tris-Bis PAGE

background

Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.

molecular weight

The protein has a predicted MW of 18.45 kDa same as Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 0.02EU per μg by the LAL method.

Human SCF Protein 2683

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
Protein names
Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
Gene names
KITLG,KITLG MGF SCF
Protein family
SCF family
Mass
9606Da
Function
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Subellular location
[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane ; Single-pass type I membrane protein. Cell projection, lamellipodium. Cell projection, filopodium .; [Soluble KIT ligand]: Secreted.
Structure
Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation.
Post-translational modification
A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.; Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.; A soluble form exists as a cleavage product of the extracellular domain.
Target Relevance information above includes information from UniProt accession: P21583
The UniProt Consortium

ELISA with Human SCF Protein
Immobilized Human SCF at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 3.42µg/ml determined by ELISA.
Cell based assay with Human SCF Protein
Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 1-8 ng/mL (QC Test).
SDS-PAGE gel of Human SCF Protein
Human SCF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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