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Human PVRIG Protein 4788

$330.00$1,100.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Thr41-Asp171
SKU: 4788parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q6DKI7

express system

HEK293

product tag

C-mFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

molecular weight

The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human PVRIG Protein 4788

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.
Protein names
Transmembrane protein PVRIG (CD112 receptor) (CD112R) (Poliovirus receptor-related immunoglobulin domain-containing protein)
Gene names
PVRIG,PVRIG C7orf15
Mass
9606Da
Function
FUNCTION: Cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding. {ECO:0000269|PubMed:26755705}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26755705}; Multi-pass membrane protein {ECO:0000305}.
Tissues
TISSUE SPECIFICITY: Expressed in some types of immune cells. Expressed at low levels on the surface of freshly isolated T-cells and natural killer (NK) cells, predominantly on CD8+ T-cells (mainly memory/effector, but not naive cells) and on both CD16+ and CD16- NK cells. T-cell expression levels are variable among individuals. Not detected in B-cells, naive or helper T-cells, monocytes, nor neutrophils (at protein level). Not detected in dendritic cells. {ECO:0000269|PubMed:26755705}.
Structure
SUBUNIT: Interacts with NECTIN2, hence competing with CD226. {ECO:0000269|PubMed:26755705}.
Target Relevance information above includes information from UniProt accession : Q6DKI7
The UniProt Consortium

Data

SPR with Human PVRIG Protein
Human PVRIG, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 µM as determined in SPR assay (Biacore T200).
ELISA with Human PVRIG Protein
Immobilized Human Nectin-2, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.17µg/ml determined by ELISA (QC Test).
ELISA with Human PVRIG Protein
Immobilized Human PVRIG, mFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
HPLC of Human PVRIG Protein
The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human PVRIG Protein
Human PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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