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Human PD-L2/B7-DC Protein 5200

$210.00$700.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Leu20-Thr220
SKU: 5200parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q9BQ51

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.

molecular weight

The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human PD-L2/B7-DC Protein 5200

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
Protein names
Programmed cell death 1 ligand 2 (PD-1 ligand 2) (PD-L2) (PDCD1 ligand 2) (Programmed death ligand 2) (Butyrophilin B7-DC) (B7-DC) (CD antigen CD273)
Protein family
Immunoglobulin superfamily, BTN/MOG family
Mass
30957Da
Function
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). {ECO:0000250}.
Subellular location
[Isoform 3]: Secreted {ECO:0000305|PubMed:15253154}.; [Isoform 2]: Endomembrane system {ECO:0000269|PubMed:15253154}; Single-pass type I membrane protein {ECO:0000255}.; [Isoform 1]: Cell membrane {ECO:0000269|PubMed:15253154}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q9WUL5, ECO:0000305|PubMed:15340161}.
Tissues
Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. {ECO:0000269|PubMed:11224527}.
Structure
Interacts with PDCD1. {ECO:0000250}.
Target Relevance information above includes information from UniProt accession: Q9BQ51
The UniProt Consortium

Data

SPR with Human PD-L2/B7-DC Protein
Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L2, His Tag with an affinity constant of 0.296 µM as determined in SPR assay (Biacore T200).
HPLC of Human PD-L2/B7-DC Protein
The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human PD-L2/B7-DC Protein
Human PD-L2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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