Human Osteoactivin/GPNMB Protein 3493

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Lys23-Asn486

SKU: 3493parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_002501
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GPNMB is highly expressed in macrophages and microglia, which are cells involved with innate immune response in the periphery and the brain, respectively. Some studies have shown increased levels of GPNMB in pro-inflammatory conditions, such as LPS treatment, and in pathological conditions, such as neurodegenerative diseases and cancer.
molecular weight	The protein has a predicted MW of 53.1 kDa. Due to glycosylation, the protein migrates to 78-98 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Osteoactivin/GPNMB Protein 3493

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GPNMB is highly expressed in macrophages and microglia, which are cells involved with innate immune response in the periphery and the brain, respectively. Some studies have shown increased levels of GPNMB in pro-inflammatory conditions, such as LPS treatment, and in pathological conditions, such as neurodegenerative diseases and cancer.
Protein names Transmembrane glycoprotein NMB (DC-HIL) (Dendritic cell-associated transmembrane protein) (Osteoactivin)
Protein family PMEL/NMB family
Mass 63676Da
Function Could be a melanogenic enzyme. {ECO:0000250}.
Subellular location Cell membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q14956}. Melanosome membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q14956}. Early endosome membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q14956}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. {ECO:0000250\|UniProtKB:Q14956}.
Tissues May be up-regulated in bone metastatic breast cancer cells. {ECO:0000269\|PubMed:17951401}.
Target Relevance information above includes information from UniProt accession: Q99P91
The UniProt Consortium

Data

HPLC of Human Osteoactivin/GPNMB Protein

The purity of Human Osteoactivin is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Osteoactivin/GPNMB Protein

Human Osteoactivin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.