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Human Nectin-1/PVRL1/CD111 Protein 4591

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Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln31-Thr334
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q15223

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.

molecular weight

The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Nectin-1/PVRL1/CD111 Protein 4591

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.
Protein names
Nectin-1 (Herpes virus entry mediator C) (Herpesvirus entry mediator C) (HveC) (Herpesvirus Ig-like receptor) (HIgR) (Nectin cell adhesion molecule 1) (Poliovirus receptor-related protein 1) (CD antigen CD111)
Gene names
NECTIN1,NECTIN1 HVEC PRR1 PVRL1
Protein family
Nectin family
Mass
9606Da
Function
FUNCTION: Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4. Has some neurite outgrowth-promoting activity. {ECO:0000269|PubMed:21980294}.; FUNCTION: (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV. {ECO:0000269|PubMed:7721102, ECO:0000269|PubMed:9657005}.
Subellular location
SUBCELLULAR LOCATION: [Isoform Alpha]: Cell membrane; Single-pass type I membrane protein. Presynaptic cell membrane {ECO:0000250}.; SUBCELLULAR LOCATION: [Isoform Delta]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform Gamma]: Secreted.
Structure
SUBUNIT: Interacts (via Ig-like C2-type domain 2) with FGFR1, FGFR2 and FGFR3 (By similarity). Cis- and trans-homodimer. Can form trans-heterodimers with NECTIN3 and with NECTIN4. Interaction between NECTIN1 and NECTIN3 on the pre- and postsynaptic sites, respectively, initiates the formation of puncta adherentia junctions between axons and dendrites. Interacts (via cytoplasmic domain) with AFDN (via PDZ domain); this interaction recruits NECTIN1 to cadherin-based adherens junctions and provides a connection with the actin cytoskeleton. Interacts with integrin alphaV/beta3. {ECO:0000250, ECO:0000269|PubMed:10225955, ECO:0000269|PubMed:11544254, ECO:0000269|PubMed:16679515, ECO:0000269|PubMed:22902367}.; SUBUNIT: (Microbial infection) Interacts with herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV envelope glycoprotein D (PubMed:21980294, PubMed:22146396, PubMed:9657005, PubMed:9696799). {ECO:0000269|PubMed:21980294, ECO:0000269|PubMed:22146396, ECO:0000269|PubMed:9657005, ECO:0000269|PubMed:9696799}.
Domain
DOMAIN: Ig
Target Relevance information above includes information from UniProt accession : Q15223
The UniProt Consortium

Data

SPR with Human Nectin-1/PVRL1/CD111 Protein
Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-1, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).
SPR with Human Nectin-1/PVRL1/CD111 Protein
Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 µM as determined in SPR assay (Biacore T200).
ELISA with Human Nectin-1/PVRL1/CD111 Protein
Immobilized Human Nectin-3, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24µg/ml determined by ELISA (QC Test).
HPLC of Human Nectin-1/PVRL1/CD111 Protein
The purity of Human Nectin-1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Nectin-1/PVRL1/CD111 Protein
Human Nectin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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