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Human MSPR/Ron Protein 2523

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Pure: Yes (SDS-PAGE)
  • Amino Acid Range: Glu25-Leu571
SKU: 2523parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

CAA49634

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE

background

Macrophage-stimulating protein (MSP) is a serum protein belonging to the plasminogen-related growth factor family. The specific receptor for MSP is the RON (recepteur d'origine nantais) receptor tyrosine kinase – a member of the MET proto-oncogene family. Activation of RON by MSP exerts dual functions on macrophages.

molecular weight

The protein has a predicted MW of 59.71 kDa. Due to furin cleavage, the protein migrates to 70-80 kDa (full length) and 40-50 kDa (alpha chain) and 35-40 kDa (partial beta chain) based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human MSPR/Ron Protein 2523

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Macrophage-stimulating protein (MSP) is a serum protein belonging to the plasminogen-related growth factor family. The specific receptor for MSP is the RON (recepteur d'origine nantais) receptor tyrosine kinase - a member of the MET proto-oncogene family. Activation of RON by MSP exerts dual functions on macrophages.
Protein names
Macrophage-stimulating protein receptor (MSP receptor) (EC 2.7.10.1) (CDw136) (Protein-tyrosine kinase 8) (p185-Ron) (CD antigen CD136) [Cleaved into: Macrophage-stimulating protein receptor alpha chain; Macrophage-stimulating protein receptor beta chain]
Protein family
Protein kinase superfamily, Tyr protein kinase family
Mass
152241Da
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand. {ECO:0000269|PubMed:18836480, ECO:0000269|PubMed:7939629, ECO:0000269|PubMed:9764835}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10028};
Subellular location
Membrane; Single-pass type I membrane protein.
Tissues
Expressed in colon, skin, lung and bone marrow. {ECO:0000269|PubMed:8062829}.
Structure
Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion. {ECO:0000269|PubMed:10222149, ECO:0000269|PubMed:10514476, ECO:0000269|PubMed:12676986, ECO:0000269|PubMed:15184888, ECO:0000269|PubMed:20726546, ECO:0000269|PubMed:21784853, ECO:0000269|PubMed:7687741}.
Post-translational modification
Proteolytic processing yields the two subunits.; Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site. {ECO:0000269|PubMed:15632155, ECO:0000269|PubMed:20726546}.; Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation. {ECO:0000269|PubMed:12802274}.; O-mannosylation of IPT/TIG domains on Thr or Ser residues by TMEM260 is required for protein maturation (PubMed:37186866). O-mannosylated residues are composed of single mannose glycans that are not elongated or modified (PubMed:37186866). {ECO:0000269|PubMed:37186866}.
Target Relevance information above includes information from UniProt accession: Q04912
The UniProt Consortium

SDS-PAGE gel of Human MSPR/Ron~-Protein
Human MSPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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