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Human MSLN/Mesothelin Protein 4422

$240.00$800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Glu296-Gly580
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q13421

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

molecular weight

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human MSLN/Mesothelin Protein 4422

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Protein names
Mesothelin (CAK1 antigen) (Pre-pro-megakaryocyte-potentiating factor) [Cleaved into: Megakaryocyte-potentiating factor (MPF); Mesothelin, cleaved form]
Gene names
MSLN,MSLN MPF
Protein family
Mesothelin family
Mass
9606Da
Function
FUNCTION: Membrane-anchored forms may play a role in cellular adhesion.; FUNCTION: Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Subellular location
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.; SUBCELLULAR LOCATION: [Megakaryocyte-potentiating factor]: Secreted.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.
Tissues
TISSUE SPECIFICITY: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). {ECO:0000269|PubMed:16857795, ECO:0000269|PubMed:7665620}.
Structure
SUBUNIT: Interacts with MUC16. {ECO:0000269|PubMed:14676194, ECO:0000269|PubMed:22787150}.
Post-translational modification
PTM: Both MPF and the cleaved form of mesothelin are N-glycosylated. {ECO:0000269|PubMed:16857795, ECO:0000269|PubMed:8288629, ECO:0000269|PubMed:8552591}.; PTM: Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. {ECO:0000269|PubMed:8552591}.
Target Relevance information above includes information from UniProt accession : Q13421
The UniProt Consortium

Data

ELISA with Human MSLN/Mesothelin Protein
Immobilized Human MSLN, His Tag at 0.5µg/ml (100µl/Well). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA.
HPLC of Human MSLN/Mesothelin Protein
The purity of Human MSLN was greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human MSLN/Mesothelin Protein
Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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