Human MADCAM1 Protein 2574

$900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln19-Gln317

SKU: 2574parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q13477
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.
molecular weight	The protein has a predicted MW of 58.17 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human MADCAM1 Protein 2574

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.
Protein names Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) (hMAdCAM-1)
Gene names MADCAM1,MADCAM1
Mass 9606Da
Function Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Structure Homodimer.
Post-translational modification The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
Target Relevance information above includes information from UniProt accession: Q13477
The UniProt Consortium

Immobilized Human MADCAM1, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA.

The purity of Human MADCAM1 is greater than 95% as determined by SEC-HPLC.

Human MADCAM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.