ELISA with Human M-CSF/CSF-1 Protein 4625

Cell based assay with Human M-CSF/CSF-1 Protein 4625

Human M-CSF/CSF-1 Protein 4625

$450.00 – $1,500.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Glu33-Asn190

SKU: 4625parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P09603
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CSF-1, also known as M-CSF, is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
molecular weight	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 28-34 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human M-CSF/CSF-1 Protein 4625

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
Protein names Macrophage colony-stimulating factor 1 (CSF-1) (M-CSF) (MCSF) (Lanimostim) (Proteoglycan macrophage colony-stimulating factor) (PG-M-CSF) [Cleaved into: Processed macrophage colony-stimulating factor 1; Macrophage colony-stimulating factor 1 43 kDa subunit]
Gene names CSF1,CSF1
Mass 9606Da
Function Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Subellular location Cell membrane ; Single-pass type I membrane protein .; [Processed macrophage colony-stimulating factor 1]: Secreted, extracellular space.
Structure Homodimer or heterodimer; disulfide-linked (PubMed:1531650, PubMed:3264877, PubMed:8422357). Likely to exist in multiple forms: homodimer consisting of 2 identical 150-200 kDa proteoglycan subunits, heterodimer consisting of a 150-200 kDa proteoglycan subunit and a truncated 43 kDa subunit, and homodimer consisting of 2 identical 43 kDa subunits (PubMed:8051056). Interacts with CSF1R (PubMed:20504948).
Post-translational modification N-glycosylated.; [Isoform 1]: N-glycosylated.; [Isoform 3]: N-glycosylated.; O-glycosylated; contains chondroitin sulfate (PubMed:1531650, PubMed:8051056). O-glycosylated with core 1 or possibly core 8 glycans (PubMed:22171320, PubMed:23234360, PubMed:3264877).; [Isoform 1]: O-glycosylated.
Target Relevance information above includes information from UniProt accession: P09603
The UniProt Consortium

Data

Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).

Immobilized Human M-CSF, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA (QC Test).

Cell based assay with Human M-CSF/CSF-1 Protein

Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 - 7.0 ng/mL.

The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human M-CSF/CSF-1 Protein

Human M-CSF on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.