ELISA with Human LY75/CD205 Protein 3439

Human LY75/CD205 Protein 3439

$300.00 – $1,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ser28-Asp1666

SKU: 3439parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O60449
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
molecular weight	The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Human LY75/CD205 Protein 3439

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
Protein names Lymphocyte antigen 75 (Ly-75) (C-type lectin domain family 13 member B) (DEC-205) (gp200-MR6) (CD antigen CD205)
Gene names LY75,LY75 CD205 CLEC13B
Mass 9606Da
Function Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
Subellular location Membrane ; Single-pass type I membrane protein .
Tissues Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
Post-translational modification N-glycosylated.
Target Relevance information above includes information from UniProt accession: O60449
The UniProt Consortium

Data

Immobilized Human LY75, His Tag at 0.1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.

The purity of Human LY75 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human LY75/CD205 Protein

Human LY75 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.