Human LRP-5 Protein 3470

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu644-Gln1263

SKU: 3470parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O75197
express system	HEK293
product tag	C-mFc
purity	> 95% as determined by Tris-Bis PAGE
background	A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.
molecular weight	The protein has a predicted MW of 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human LRP-5 Protein 3470

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.
Protein names Low-density lipoprotein receptor-related protein 5 (LRP-5) (Low-density lipoprotein receptor-related protein 7) (LRP-7)
Protein family 248
Function Acts as a coreceptor with members of the frizzled family of seven-transmembrane spanning receptors to transduce signal by Wnt proteins (PubMed:11336703, PubMed:11448771, PubMed:11719191, PubMed:15778503, PubMed:15908424, PubMed:16252235). Activates the canonical Wnt signaling pathway that controls cell fate determination and self-renewal during embryonic development and adult tissue regeneration (PubMed:11336703, PubMed:11719191). In particular, may play an important role in the development of the posterior patterning of the epiblast during gastrulation (By similarity). During bone development, regulates osteoblast proliferation and differentiation thus determining bone mass (PubMed:11719191). Mechanistically, the formation of the signaling complex between Wnt ligand, frizzled receptor and LRP5 coreceptor promotes the recruitment of AXIN1 to LRP5, stabilizing beta-catenin/CTNNB1 and activating TCF/LEF-mediated transcriptional programs (PubMed:11336703, PubMed:14731402, PubMed:24706814, PubMed:25920554). Acts as a coreceptor for non-Wnt proteins, such as norrin/NDP. Binding of norrin/NDP to frizzled 4/FZD4-LRP5 receptor complex triggers beta-catenin/CTNNB1-dependent signaling known to be required for retinal vascular development (PubMed:16252235, PubMed:27228167). Plays a role in controlling postnatal vascular regression in retina via macrophage-induced endothelial cell apoptosis (By similarity).
Pathway 142
Subellular location Membrane ; Single-pass type I membrane protein. Endoplasmic reticulum. Note=Chaperoned to the plasma membrane by MESD.
Tissues Widely expressed, with the highest level of expression in the liver and in aorta.
Structure Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling (By similarity). Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts with FZD8; the interaction is formed on WNT-binding and signaling (PubMed:11448771). Interacts (via the phosphorylated PPPSP motif domains) with AXIN1; the interaction prevents inhibition of beta-catenin phosphorylation and signaling and is enhanced in the presence of GSK3B and WNT1 or WNT3A (PubMed:11336703, PubMed:14731402). Interacts (via beta-propeller regions 3 and 4) with DKK1; the interaction, enhanced by MESD and/or KREMEN, inhibits beta-catenin signaling by preventing GSK3-mediated phosphorylation of the PPPSP motifs and subsequent, AXIN1 binding (PubMed:11448771, PubMed:15778503, PubMed:19746449). Interacts with MESD; the interaction prevents the formation of LRP5 aggregates, targets LRP5 to the plasma membrane and, when complexed with KREMEN2, increases DKK1 binding (PubMed:15143163, PubMed:17488095, PubMed:19746449). Interacts with CSNK1E (PubMed:16513652). Interacts with SOST; the interaction antagonizes canonical Wnt signaling (PubMed:15778503, PubMed:15908424). Interacts with APCDD1 (PubMed:20393562). Interacts with CAPRIN2 (PubMed:18762581).
Post-translational modification Phosphorylation of cytoplasmic PPPSP motifs regulates the signal transduction of the Wnt signaling pathway through acting as a docking site for AXIN1.
Target Relevance information above includes information from UniProt accession: O75197
The UniProt Consortium

Immobilized Human Wnt Surrogate-Fc Fusion, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.73µg/ml determined by ELISA (QC Test).

Immobilized Human DKK1, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.11µg/ml determined by ELISA.

Human LRP-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. Question: What is the source of the Human LRP-5 Protein 3470?
Answer: The Human LRP-5 Protein 3470 is expressed using a mammalian system, ensuring proper protein folding and post-translational modifications for accurate functionality.

2. Question: How should I store the Human LRP-5 Protein 3470 upon receiving it?
Answer: We recommend storing the protein at -80°C upon arrival to maintain its stability and activity. Avoid repeated freeze-thaw cycles for best results.

3. Question: Is the Human LRP-5 Protein 3470 compatible with a variety of applications?
Answer: Yes, the Human LRP-5 Protein 3470 is suitable for various applications including Western blotting, ELISA, immunoprecipitation, and functional studies in cell culture systems.

4. Question: Can I use this protein for in vivo experiments or animal studies?
Answer: This protein is intended for in vitro research purposes only and is not recommended for use in vivo or in animal studies.

5. Question: Does the Human LRP-5 Protein 3470 come with any quality control documentation?
Answer: Yes, each batch of the Human LRP-5 Protein 3470 undergoes rigorous quality control testing to ensure purity and activity, and a certificate of analysis is provided with each purchase for your reference.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.