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Human LIV-1/SLC39A6 Protein 2010

$375.00$1,250.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Phe29-Trp325
SKU: 2010parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q13433

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Zinc transporter ZIP6 (SLC39A6) or LIV-1 is a protein that belongs to a subfamily of proteins group that displays structural specifications of zinc transporters in the cell membrane. Overexpression of this protein is observed in breast, prostate, and kidney tumor cells. Lately, LIV-1 is a dependable marker for detection of estrogen receptor positive breast cancer, which can be used to detect luminal breast cancer type A.

molecular weight

The protein has a predicted MW of 60.39 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human LIV-1/SLC39A6 Protein 2010

protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Zinc transporter ZIP6 (SLC39A6) or LIV-1 is a protein that belongs to a subfamily of proteins group that displays structural specifications of zinc transporters in the cell membrane. Overexpression of this protein is observed in breast, prostate, and kidney tumor cells. Lately, LIV-1 is a dependable marker for detection of estrogen receptor positive breast cancer, which can be used to detect luminal breast cancer type A.
Protein names
Zinc transporter ZIP6 (Estrogen-regulated protein LIV-1) (Solute carrier family 39 member 6) (Zrt- and Irt-like protein 6) (ZIP-6)
Gene names
SLC39A6,SLC39A6 LIV1 ZIP6
Protein family
ZIP transporter (TC 2.A.5) family
Mass
9606Da
Function
Zinc-influx transporter which plays a role in zinc homeostasis and in the induction of epithelial-to-mesenchymal transition (EMT) (PubMed:12839489, PubMed:18272141, PubMed:21422171, PubMed:23919497, PubMed:27274087, PubMed:34394081). When associated with SLC39A10, the heterodimer formed by SLC39A10 and SLC39A6 mediates cellular zinc uptake to trigger cells to undergo epithelial- to-mesenchymal transition (EMT) (PubMed:27274087). The SLC39A10-SLC39A6 heterodimer also controls NCAM1 phosphorylation and its integration into focal adhesion complexes during EMT (By similarity). Zinc influx inactivates GSK3B, enabling unphosphorylated SNAI1 in the nucleus to down-regulate adherence genes such as CDH1, causing loss of cell adherence (PubMed:23919497). In addition, the SLC39A10-SLC39A6 heterodimer plays an essentiel role in initiating mitosis by importing zinc into cells to initiate a pathway resulting in the onset of mitosis (PubMed:32797246). Participates in the T-cell receptor signaling regulation by mediating cellular zinc uptake into activated lymphocytes (PubMed:21422171, PubMed:30552163, PubMed:34394081). Regulates the zinc influx necessary for proper meiotic progression to metaphase II (MII) that allows the oocyte-to-egg transition (PubMed:25143461).
Subellular location
Cell membrane ; Multi-pass membrane protein. Cell projection, lamellipodium membrane ; Multi-pass membrane protein. Membrane raft ; Multi-pass membrane protein. Apical cell membrane. Note=Localizes to lipid rafts in T cells and is recruited into the immunological synapse in response to TCR stimulation (PubMed:34394081). In the choroid plexus is limited to the apical membrane in epithelial cells (By similarity).
Tissues
Highly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum (PubMed:12839489). Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma (PubMed:12839489).
Structure
Interacts with SLC39A10; which triggers cells to undergo EMT and mitosis (PubMed:27274087). Found in a complex with SLC39A6, SLC39A10 and with the 'Ser-727' phosphorylated form of STAT3 throughout mitosis (PubMed:32797246). Found in a complex with SLC39A6, SLC39A10 and with NCAM1; this complex controls NCAM1 phosphorylation and integration into focal adhesion complexes during epithelial-to-mesenchymal transition (EMT) (By similarity). Found in a complex with SLC39A6, SLC39A10 and with GSK3B that controls NCAM1 phosphorylation (By similarity).
Post-translational modification
Cleaved on the N-terminus before locating to the plasma membrane.; N-glycosylated.; Phosphorylated by ZAP70 in response to TCR stimulation leading to its activation.
Target Relevance information above includes information from UniProt accession: Q13433
The UniProt Consortium

ELISA with Human LIV-1/SLC39A6 Protein
Immobilized Human LIV-1, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-LIV-1 Antibody, hFc Tag with the EC50 of 46.7ng/ml determined by ELISA.
HPLC of Human LIV-1/SLC39A6 Protein
The purity of Human LIV-1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human LIV-1/SLC39A6 Protein
Human LIV-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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