ELISA with Human LILRB3/CD85a/ILT5 Protein 4606

Human LILRB3/CD85a/ILT5 Protein 4606

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly24-Glu443

SKU: 4606parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O75022
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.
molecular weight	The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human LILRB3/CD85a/ILT5 Protein 4606

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.
Protein names Leukocyte immunoglobulin-like receptor subfamily B member 3 (LIR-3) (Leukocyte immunoglobulin-like receptor 3) (CD85 antigen-like family member A) (Immunoglobulin-like transcript 5) (ILT-5) (Monocyte inhibitory receptor HL9) (CD antigen CD85a)
Gene names LILRB3,LILRB3 ILT5 LIR3
Mass 9606Da
Function May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).
Subellular location Cell membrane; Single-pass type I membrane protein.
Tissues Detected in monocytes and B-cells.
Structure Interacts with LYN, PTPN6/SHP-1 and PTPN11/SHP-2.
Post-translational modification Phosphorylated on tyrosine residues by LYN. Phosphorylation at Tyr-595 and Tyr-625 is important for interaction with PTPN6/SHP-1 and PTPN11/SHP-2.
Domain Co
Target Relevance information above includes information from UniProt accession: O75022
The UniProt Consortium

Data

ELISA with Human LILRB3/CD85a/ILT5 Protein

Immobilized Human LILRB3, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA.

The purity of Human LILRB3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human LILRB3/CD85a/ILT5 Protein

Human LILRB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.