SPR with Human LILRB2/CD85d/ILT4 Domain1&2 Protein 4319

ELISA with Human LILRB2/CD85d/ILT4 Domain1&2 Protein 4319

Binding assay with Human LILRB2/CD85d/ILT4 Domain1&2 Protein 4319

Human LILRB2/CD85d/ILT4 Domain1&2 Protein 4319

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (binding assay)
Amino Acid Range: Gln22-Val229

SKU: 4319parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q8N423
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
molecular weight	The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human LILRB2/CD85d/ILT4 Domain1&2 Protein 4319

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Protein names Leukocyte immunoglobulin-like receptor subfamily B member 2 (LIR-2) (Leukocyte immunoglobulin-like receptor 2) (CD85 antigen-like family member D) (Immunoglobulin-like transcript 4) (ILT-4) (Monocyte/macrophage immunoglobulin-like receptor 10) (MIR-10) (CD antigen CD85d)
Gene names LILRB2,LILRB2 ILT4 LIR2 MIR10
Mass 9606Da
Function Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885).
Subellular location Cell membrane ; Single-pass type I membrane protein.
Tissues Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells (PubMed:20448110). Expressed in myeloid-derived suppressor cells during pregnancy (PubMed:27859042). Detected at low levels in natural killer (NK) cells. Expressed in B cells.
Structure Binds PTPN6 when phosphorylated. Binds FCGR1A. Interacts with peptide-bound HLA-G-B2M; this interaction is direct (PubMed:16455647). Interacts with peptide-bound HLA-F-B2M; this interaction is direct (PubMed:11169396).
Post-translational modification Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.
Domain Co
Target Relevance information above includes information from UniProt accession: Q8N423
The UniProt Consortium

Data

SPR with Human LILRB2/CD85d/ILT4 Domain1&2 Protein

Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5µM as determined in a SPR assay (Biacore T200).

ELISA with Human LILRB2/CD85d/ILT4 Domain1&2 Protein

Immobilized Human LILRB2 Domain1&2, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.6ng/ml determined by ELISA (QC Test).

Binding assay with Human LILRB2/CD85d/ILT4 Domain1&2 Protein

Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43µg/ml.

HPLC of Human LILRB2/CD85d/ILT4 Domain1&2 Protein

The purity of Human LILRB2 Domain 1&2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human LILRB2/CD85d/ILT4 Domain1&2 Protein

Human LILRB2 Domain 1&2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.