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Human LIGHT/TNFSF14 Trimer Protein 4461

$270.00$900.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ser89-Val240
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

O43557

express system

HEK293

product tag

N-His-Flag

purity

> 90% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.

molecular weight

The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human LIGHT/TNFSF14 Trimer Protein 4461

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Protein names
Tumor necrosis factor ligand superfamily member 14 (Herpes virus entry mediator ligand) (HVEM-L) (Herpesvirus entry mediator ligand) (CD antigen CD258) [Cleaved into: Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form]
Gene names
TNFSF14,TNFSF14 HVEML LIGHT UNQ391/PRO726
Protein family
Tumor necrosis factor family
Mass
9606Da
Function
FUNCTION: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM (PubMed:10754304, PubMed:9462508). Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production (PubMed:10754304). {ECO:0000269|PubMed:10754304, ECO:0000269|PubMed:9462508}.
Subellular location
SUBCELLULAR LOCATION: [Tumor necrosis factor ligand superfamily member 14, membrane form]: Cell membrane; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Tumor necrosis factor ligand superfamily member 14, soluble form]: Secreted.; SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm.
Tissues
TISSUE SPECIFICITY: Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines. {ECO:0000269|PubMed:9462508}.
Structure
SUBUNIT: Homotrimer. Interacts with TNFRSF14. {ECO:0000269|PubMed:9462508}.
Post-translational modification
PTM: N-glycosylated. {ECO:0000269|Ref.10}.; PTM: The soluble form of isoform 1 derives from the membrane form by proteolytic processing. {ECO:0000269|PubMed:11673523}.
Target Relevance information above includes information from UniProt accession : O43557
The UniProt Consortium

Data

ELISA with Human LIGHT/TNFSF14 Trimer Protein
Immobilized Human LIGHT Trimer, His tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 86.3ng/ml determined by ELISA.
HPLC of Human LIGHT/TNFSF14 Trimer Protein
The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human LIGHT/TNFSF14 Trimer Protein
Human LIGHT Trimer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 90%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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