ELISA with Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Prot 3262

SDS-PAGE gel of Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer 3262

Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein 3262

$375.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe20-Pro1107(ITGAX) acidic tail & Gln23-Asn700(ITGB2) basic tail

SKU: 3262parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P20702(ITGAX)&P05107(ITGB2)
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The integrin alpha X beta 2 (CD11c/CD18, p150, 95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response.
molecular weight	The protein has a predicted MW of 125.50 kDa(ITGAX)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 130-135 kDa(ITGAX)&85-95 kDa(ITGB2) kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein 3262

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response.
Protein names Integrin alpha-X (CD11 antigen-like family member C) (Leu M5) (Leukocyte adhesion glycoprotein p150,95 alpha chain) (Leukocyte adhesion receptor p150,95) (CD antigen CD11c)
Gene names ITGAX,ITGAX CD11C
Protein family Integrin alpha chain family
Mass 127829Da
Function Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Predominantly expressed in monocytes and granulocytes.
Structure Heterodimer of an alpha and a beta subunit. Alpha-X associates with beta-2.
Domain The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo pro
Target Relevance information above includes information from UniProt accession: P20702
The UniProt Consortium

Data

ELISA with Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Prot

Immobilized Human ITGAX&ITGB2, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA.

HPLC of Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein

The purity of Human ITGAX&ITGB2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer

Human ITGAX&ITGB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.