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Human IL-7R alpha/CD127 Protein 4893

$225.00$750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Glu21-Gly236
SKU: 4893parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P16871

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.

molecular weight

The protein has a predicted MW of 51.6 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-7R alpha/CD127 Protein 4893

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.
Protein names
Interleukin-7 receptor subunit alpha (IL-7 receptor subunit alpha) (IL-7R subunit alpha) (IL-7R-alpha) (IL-7RA) (CDw127) (CD antigen CD127)
Protein family
Type I cytokine receptor family, Type 4 subfamily
Mass
51579Da
Function
Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Subellular location
[Isoform 1]: Cell membrane; Single-pass type I membrane protein {ECO:0000269|PubMed:31748347}.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Secreted.
Structure
The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R. Interacts with CD53 (PubMed:31748347). {ECO:0000269|PubMed:19141282, ECO:0000269|PubMed:31748347}.
Post-translational modification
N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. {ECO:0000269|PubMed:19141282}.
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient int
Target Relevance information above includes information from UniProt accession: P16871
The UniProt Consortium

Data

ELISA with Human IL-7R alpha/CD127 Protein
Immobilized Human IL-7 at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
HPLC of Human IL-7R alpha/CD127 Protein
The purity of Human IL-7R alpha is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-7R alpha/CD127 Protein
Human IL-7R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

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