ELISA with Human IL-3 R alpha/CD123 Protein 5180

Human IL-3 R alpha/CD123 Protein 5180

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Thr19-Arg305

SKU: 5180parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P26951
express system	HEK293
product tag	C-hFc
purity	> 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
molecular weight	The protein has a predicted MW of 59.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-3 R alpha/CD123 Protein 5180

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
Protein names Interleukin-3 receptor subunit alpha (IL-3 receptor subunit alpha) (IL-3R subunit alpha) (IL-3R-alpha) (IL-3RA) (CD antigen CD123)
Gene names IL3RA,IL3RA IL3R
Protein family Type I cytokine receptor family, Type 5 subfamily
Mass 9606Da
Function Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed:10527461). Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity).
Subellular location Membrane; Single-pass type I membrane protein.
Structure Interacts with IL3 (PubMed:29374162). Heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors.
Post-translational modification Ubiquitinated by RNFT2 in response to IL3. Ubiquitination leads ligand-induced degradation by the proteasome.
Domain Th
Target Relevance information above includes information from UniProt accession: P26951
The UniProt Consortium

Data

ELISA with Human IL-3 R alpha/CD123 Protein

Immobilized Human IL-3, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46µg/ml determined by ELISA.

HPLC of Human IL-3 R alpha/CD123 Protein

The purity of Human IL-3 R alpha is greater than 90% as determined by SEC-HPLC.

SDS-PAGE gel of Human IL-3 R alpha/CD123 Protein

Human IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.