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Human IL-3 R alpha/CD123 Protein 4460

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Thr19-Arg305
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P26951

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE

background

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.

molecular weight

The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-3 R alpha/CD123 Protein 4460

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
Protein names
Interleukin-3 receptor subunit alpha (IL-3 receptor subunit alpha) (IL-3R subunit alpha) (IL-3R-alpha) (IL-3RA) (CD antigen CD123)
Gene names
IL3RA,IL3RA IL3R
Protein family
Type I cytokine receptor family, Type 5 subfamily
Mass
9606Da
Function
FUNCTION: Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed:10527461). Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity). {ECO:0000250|UniProtKB:P26952, ECO:0000269|PubMed:10527461, ECO:0000269|PubMed:29374162}.
Subellular location
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
Structure
SUBUNIT: Interacts with IL3 (PubMed:29374162). Heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors.
Post-translational modification
PTM: Ubiquitinated by RNFT2 in response to IL3. Ubiquitination leads ligand-induced degradation by the proteasome. {ECO:0000250|UniProtKB:P26952}.
Domain
DOMAIN: Th
Target Relevance information above includes information from UniProt accession : P26951
The UniProt Consortium

Data

SPR with Human IL-3 R alpha/CD123 Protein
Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 µM as determined in SPR assay (Biacore T200).
ELISA with Human IL-3 R alpha/CD123 Protein
Immobilized Human IL-3 R alpha, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).
HPLC of Human IL-3 R alpha/CD123 Protein
The purity of Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-3 R alpha/CD123 Protein
Human IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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