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Human IL-27 Protein 2740

$315.00$1,050.00

Summary

  • Expression: CHO
  • Functional: Yes (ELISA)
  • Amino Acid Range: Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A)
SKU: 2740parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q14213(IL7B)&Q8NEV9(IL7A)

express system

CHO

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE

background

Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.

molecular weight

The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-27 Protein 2740

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.
Protein names
Interleukin-27 subunit beta (IL-27 subunit beta) (IL-27B) (Epstein-Barr virus-induced gene 3 protein) (EBV-induced gene 3 protein)
Gene names
EBI3,EBI3 IL27B
Protein family
Type I cytokine receptor family, Type 3 subfamily
Mass
25396Da
Function
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Subellular location
Secreted .
Structure
Heterodimer with IL27/IL27A; not disulfide-linked (PubMed:12121660). This heterodimer is known as interleukin IL-27 (PubMed:12121660). Heterodimer with IL12A; not disulfide-linked (PubMed:9342359). This heterodimer is known as interleukin IL-35 (PubMed:9342359). Interacts with SQSTM1 (PubMed:8551575).
Target Relevance information above includes information from UniProt accession: Q14213
The UniProt Consortium

SPR with Human IL-27 Protein
Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
ELISA with Human IL-27 Protein
Immobilized Human IL-27 RA, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).
SDS-PAGE gel of Human IL-27 Protein
Human IL-27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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