ELISA with Human IL-22R alpha 1&IL-10R beta Protein 3211

Human IL-22R alpha 1&IL-10R beta Protein 3211

$450.00 – $1,500.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta)

SKU: 3211parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q8N6P7(IL2R alpha 1)&Q08334(IL0R beta)
express system	HEK293
product tag	C-hFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	IL-22Rα1 & IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL-22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL10 Rβ or IL20 Rβ and the respective ligands is required for signal transduction.
molecular weight	The protein has a predicted MW of 52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-22R alpha 1&IL-10R beta Protein 3211

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
IL-22Rα1 & IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. ILÂ-22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with ILÂ10 Rβ or ILÂ20 Rβ and the respective ligands is required for signal transduction.
Protein names Interleukin-22 receptor subunit alpha-1 (IL-22 receptor subunit alpha-1) (IL-22R-alpha-1) (IL-22RA1) (Cytokine receptor class-II member 9) (Cytokine receptor family 2 member 9) (CRF2-9) (ZcytoR11)
Gene names IL22RA1,IL22RA1 IL22R
Protein family Type II cytokine receptor family
Mass 63077Da
Function Component of the receptor for IL20, IL22 and IL24. Component of IL22 receptor formed by IL22RA1 and IL10RB enabling IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. Mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed in colon, liver, lung, pancreas and kidney. No expression in immune cells such as monocytes, T-cells, and NK-cells. Expressed in keratinocytes of normal skin as well as in psoriatic skin lesion. Detected in normal blood brain barrier endothelial cells as well as in multiple sclerosis lesions; Strongly expressed on central nervous system vessels within infiltrated multiple sclerosis lesions. Overexpressed in synovial fluid cells from rheumatoid arthritis and spondyloarthropathy patients.
Structure Heterodimer with IL10RB and with IL20RB. IL22 binding to heterodimer is greater than binding to IL22RA1 alone (PubMed:11035029, PubMed:15120653, PubMed:18675809). Interacts with FBXW12; the interaction promotes ubiquitination of IL22RA1 (PubMed:26171402).
Post-translational modification Ubiquitinated.
Domain TOPO_DOM 1
Target Relevance information above includes information from UniProt accession: Q8N6P7
The UniProt Consortium

ELISA with Human IL-22R alpha 1&IL-10R beta Protein

Immobilized Human IL-22, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-22R alpha 1&IL-10R beta, hFc Tag with the EC50 of 0.24µg/ml determined by ELISA.

HPLC of Human IL-22R alpha 1&IL-10R beta Protein

The purity of Human IL-22R alpha 1&IL-10R beta is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human IL-22R alpha 1&IL-10R beta Protein

Human IL-22R alpha 1&IL-10R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. Question: What is the concentration of the Human IL-22R alpha-1/IL-10R beta protein product?
Answer: The concentration of the Human IL-22R alpha-1/IL-10R beta protein is 0.5 mg/ml.

2. Question: How should the Human IL-22R alpha-1/IL-10R beta protein be stored?
Answer: It is recommended to store this protein at -20°C upon arrival. Avoid repeated freeze-thaw cycles to maintain its stability.

3. Question: Is this Human IL-22R alpha-1/IL-10R beta protein compatible with a variety of applications?
Answer: Yes, this protein is suitable for use in various applications such as ELISA, Western blot, and other functional assays.

4. Question: What is the purity level of the Human IL-22R alpha-1/IL-10R beta protein?
Answer: The purity of the Human IL-22R alpha-1/IL-10R beta protein is greater than 90%.

5. Question: Can this Human IL-22R alpha-1/IL-10R beta protein be used in cell culture experiments?
Answer: Yes, this protein can be used in cell culture experiments to study its interaction with other molecules and signaling pathways.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.