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Human IL-2 R beta/CD122 Protein 4636

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ala27-Asp239
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

NP_000869

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.

molecular weight

The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-2 R beta/CD122 Protein 4636

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

SPR with Human IL-2 R beta/CD122 Protein
Human IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.12 µM as determined in SPR assay (Biacore T200).
SPR with Human IL-2 R beta/CD122 Protein
Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9nM as determined in SPR assay (Biacore T200).
ELISA with Human IL-2 R beta/CD122 Protein
Immobilized Human IL-2 R beta, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, hFc Tag with the EC50 of 43.7ng/ml determined by ELISA (QC Test).
HPLC of Human IL-2 R beta/CD122 Protein
The purity of Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-2 R beta/CD122 Protein
Human IL-2 R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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