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Human IL-2 R alpha/CD25 Protein 4502

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Glu22-Cys213
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P01589

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

molecular weight

The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-2 R alpha/CD25 Protein 4502

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Protein names
Interleukin-2 receptor subunit alpha (IL-2 receptor subunit alpha) (IL-2-RA) (IL-2R subunit alpha) (IL2-RA) (TAC antigen) (p55) (CD antigen CD25)
Gene names
IL2RA,IL2RA
Mass
9606Da
Function
FUNCTION: Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. {ECO:0000269|PubMed:23416241, ECO:0000269|PubMed:24116927}.
Subellular location
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
Structure
SUBUNIT: Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. {ECO:0000269|PubMed:16293754, ECO:0000269|PubMed:16477002}.
Target Relevance information above includes information from UniProt accession : P01589
The UniProt Consortium

Data

SPR with Human IL-2 R alpha/CD25 Protein
Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-2, No Tag with an affinity constant of 15.2nM as determined in SPR assay (Biacore T200).
ELISA with Human IL-2 R alpha/CD25 Protein
Immobilized Human IL-2 R alpha, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
ELISA with Human IL-2 R alpha/CD25 Protein
Immobilized Human IL-2 R alpha, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human IL-2, His Tag with the EC50 of 59.6ng/ml determined by ELISA.
HPLC of Human IL-2 R alpha/CD25 Protein
The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-2 R alpha/CD25 Protein
Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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