ELISA with Human IL-1RL1/ST2 Protein 2365

Human IL-1RL1/ST2 Protein 2365

$135.00 – $450.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Lys19-Phe328

SKU: 2365parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q01638
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	IL1RL1(also known as ST2) is a member of the IL1 receptor family and serves as the receptor for IL-33. The ST2 protein has two isoforms including sST2 and the membrane-bound ST2 receptor, referred to as ST2L. Preclinical studies have demonstrated that binding of IL-33 to ST2L elicits a cardioprotective effect. ST2/IL-33 signaling may play an important role in intestinal disease.
molecular weight	The protein has a predicted MW of 61.75 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-1RL1/ST2 Protein 2365

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
IL1RL1(also known as ST2)Â is a member of the IL1Â receptor familyÂ and serves as the receptor for IL-33. TheÂ ST2 proteinÂ has two isoforms including sST2 and the membrane-bound ST2 receptor, referred to as ST2L. Preclinical studies have demonstrated that binding of IL-33 to ST2L elicits a cardioprotective effect. ST2/IL-33 signaling may play an important role in intestinal disease.
Protein names Interleukin-1 receptor-like 1 (EC 3.2.2.6) (Protein ST2)
Gene names IL1RL1,IL1RL1 DER4 ST2 T1
Protein family Interleukin-1 receptor family
Mass 9606Da
Function Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed:35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed:16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).; [Isoform B]: Inhibits IL-33 signaling.
Subellular location [Isoform C]: Cell membrane.; [Isoform B]: Secreted.; Cell membrane ; Single-pass type I membrane protein .
Tissues Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. Expressed on T-cells in fibrotic liver; at protein level. Overexpressed in fibrotic and cirrhotic liver.
Structure Interacts with MYD88, IRAK1, IRAK4, and TRAF6. Bound to its ligand IL-33, interacts with IL1RAP to form the minimal interleukin-33 signaling complex with a 1:1:1 stoichiometry. Interacts with KIT (bound to KITLG/SCF). A mast cell-specific KITLG/SCF-induced interleukin-33 signaling complex contains IL1RL1, IL1RAP, KIT and MYD88. Interacts with TMED1 (PubMed:23319592).
Post-translational modification Ubiquitinated at Lys-321 in a FBXL19-mediated manner; leading to proteasomal degradation. Ubiquitination by TRAF6 via 'Lys-27'-linked polyubiquitination and deubiquitination by USP38 serves as a critical regulatory mechanism for fine-tuning IL1RL1-mediated inflammatory response (PubMed:35238669).
Domain Th
Target Relevance information above includes information from UniProt accession: Q01638
The UniProt Consortium

Data

Immobilized Human IL-33, No Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-1RL1, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

The purity of Human IL-1RL1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human IL-1RL1/ST2 Protein

Human IL-1RL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.