ELISA with Human IL-1R2/IL-1 RII/CD121b Protein 4703

Human IL-1R2/IL-1 RII/CD121b Protein 4703

$210.00 – $700.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe14-Glu343

available size

Clear

SKU: 4703parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_004624
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family.IL1R2 (Interleukin 1 Receptor Type 2) is a Protein Coding gene. Diseases associated with IL1R2 include Endometriosis and Mastitis. Among its related pathways are Hematopoietic cell lineage and IL-1 signaling pathway. Gene Ontology (GO) annotations related to this gene include interleukin-1 receptor activity and interleukin-1, type II, blocking receptor activity.
molecular weight	The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-1R2/IL-1 RII/CD121b Protein 4703

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family.IL1R2 (Interleukin 1 Receptor Type 2) is a Protein Coding gene. Diseases associated with IL1R2 include Endometriosis and Mastitis. Among its related pathways are Hematopoietic cell lineage and IL-1 signaling pathway. Gene Ontology (GO) annotations related to this gene include interleukin-1 receptor activity and interleukin-1, type II, blocking receptor activity.
Protein names GTPase KRas (EC 3.6.5.2) (K-Ras 2) (Ki-Ras) (c-K-ras) (c-Ki-ras) [Cleaved into: GTPase KRas, N-terminally processed]
Gene names KRAS,KRAS KRAS2 RASK2
Protein family Small GTPase superfamily, Ras family
Mass 21656Da
Function FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621). Plays an important role in the regulation of cell proliferation (PubMed:22711838, PubMed:23698361). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306). {ECO:0000269\|PubMed:20949621, ECO:0000269\|PubMed:22711838, ECO:0000269\|PubMed:23698361, ECO:0000269\|PubMed:24623306, ECO:0000305}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=GTP + H2O = GDP + phosphate + H(+); Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2; Evidence={ECO:0000269\|PubMed:20949621};
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:22431598, ECO:0000269\|PubMed:23698361, ECO:0000269\|PubMed:29239724}; Lipid-anchor {ECO:0000269\|PubMed:29239724, ECO:0000305\|PubMed:23698361}; Cytoplasmic side {ECO:0000305\|PubMed:23698361}. Endomembrane system {ECO:0000269\|PubMed:29239724}. Cytoplasm, cytosol {ECO:0000269\|PubMed:23698361}.; SUBCELLULAR LOCATION: [Isoform 2B]: Cell membrane {ECO:0000269\|PubMed:28619714}; Lipid-anchor {ECO:0000305\|PubMed:28619714}.
Structure SUBUNIT: Interacts with PHLPP (By similarity). Interacts (active GTP-bound form preferentially) with RGS14 (By similarity). Interacts (when farnesylated) with PDE6D; this promotes dissociation from the cell membrane (PubMed:23698361). Interacts with SOS1 (PubMed:22431598). Interacts (when farnesylated) with GPR31 (PubMed:28619714). Interacts with RAP1GDS1 (PubMed:20709748, PubMed:24415755). Interacts (active GTP-bound form) with both SHOC2 and PP1c (all isoforms) to form a tertiary complex; SHOC2 and PP1c preferably bind M-Ras/MRAS, but they also bind K-Ras/KRAS, N-Ras/NRAS and H-Ras/HRAS (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). Interacts (GTP-bound form) with MAPKAP1/SIN1; inhibiting K-Ras/KRAS activity (PubMed:34380736, PubMed:35522713). {ECO:0000250\|UniProtKB:P08644, ECO:0000269\|PubMed:20709748, ECO:0000269\|PubMed:22431598, ECO:0000269\|PubMed:23698361, ECO:0000269\|PubMed:24415755, ECO:0000269\|PubMed:28619714, ECO:0000269\|PubMed:34380736, ECO:0000269\|PubMed:35522713, ECO:0000269\|PubMed:35768504, ECO:0000269\|PubMed:35830882, ECO:0000269\|PubMed:35831509, ECO:0000269\|PubMed:36175670}.; SUBUNIT: [Isoform 2B]: Interacts with GPR31; in a farnelysation-dependent manner. {ECO:0000269\|PubMed:28619714}.
Post-translational modification PTM: Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). {ECO:0000269\|PubMed:22711838, ECO:0000269\|Ref.17}.; PTM: Palmitoylated at Lys-182, Lys-184 and Lys-185 (PubMed:29239724). Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180 (PubMed:29239724). Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways (PubMed:29239724). {ECO:0000269\|PubMed:29239724}.; PTM: Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. {ECO:0000305\|PubMed:30442762, ECO:0000305\|PubMed:30442766}.; PTM: (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. {ECO:0000269\|PubMed:19744486}.
Target Relevance information above includes information from UniProt accession: P01116
The UniProt Consortium

Data

ELISA with Human IL-1R2/IL-1 RII/CD121b Protein

Immobilized Human IL-1R2, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 30.9ng/ml determined by ELISA.

HPLC of Human IL-1R2/IL-1 RII/CD121b Protein

The purity of Human IL-1R2 is greater than 90% as determined by SEC-HPLC.

SDS-PAGE gel of Human IL-1R2/IL-1 RII/CD121b Protein

Human IL-1R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.