SPR with Human IL-1R2/IL-1 RII/CD121b Protein 4240

Human IL-1R2/IL-1 RII/CD121b Protein 4240

$225.00 – $750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe14-Glu343

SKU: 4240parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_004624
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE
background	The IL-1 type II receptor (decoy RII) is a nonsignaling molecule the only established function of which is to capture IL-1 and prevent it from interacting with signaling receptor. The decoy RII is released in a regulated way from the cell surface.
molecular weight	The protein has a predicted MW of 64.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-1R2/IL-1 RII/CD121b Protein 4240

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

SPR with Human IL-1R2/IL-1 RII/CD121b Protein

Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).

ELISA with Human IL-1R2/IL-1 RII/CD121b Protein

Immobilized Human IL-1 Beta, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12µg/ml determined by ELISA (QC Test).

SDS-PAGE gel of Human IL-1R2/IL-1 RII/CD121b Protein

Human IL-1R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.