Cell based assay with Human IL-15 Protein 4753

Human IL-15 Protein 4753

$255.00 – $850.00

Summary

Expression: E.coli
Active: Yes (cell based assay)
Amino Acid Range: Asn49-Ser162

SKU: 4753parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P40933
express system	E.coli
product tag	No Tag
purity	>95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
background	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
molecular weight	The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 0.05 EU per μg by the LAL method.

Human IL-15 Protein 4753

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
Protein names Interleukin-15 (IL-15)
Gene names IL15,IL15
Protein family IL-15/IL-21 family
Mass 18086Da
Function Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed:15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed:8178155, PubMed:9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed:9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed:10233906, PubMed:23104097, PubMed:8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed:7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
Subellular location [Isoform IL15-S48AA]: Secreted.; [Isoform IL15-S21AA]: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
Tissues Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
Target Relevance information above includes information from UniProt accession: P40933
The UniProt Consortium

Data

Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-15, No Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4ng/ml determined by ELISA.

Cell based assay with Human IL-15 Protein

Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 x 10^7 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).

The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.

Human IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.