SPR with Human IL-12B/p40/NKSF2 Protein 4292

ELISA with Human IL-12B/p40/NKSF2 Protein 4292

Human IL-12B/p40/NKSF2 Protein 4292

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ile23-Ser328

SKU: 4292parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P29460
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE
background	Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
molecular weight	The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-12B/p40/NKSF2 Protein 4292

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin (IL)-12B, which encodes the p40 subunit common to IL-12 and IL-23, as one of the genes for which expression in fibroblast-like synoviocytes from patients with rheumatoid arthritis (RA-FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin-related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor-like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
Protein names Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40) (NK cell stimulatory factor chain 2) (NKSF2)
Gene names IL12B,IL12B NKSF2
Protein family IL-12B family
Mass 9606Da
Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Subellular location Secreted.
Structure Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer. Interacts with NBR1; this interaction promotes IL-12 secretion (By similarity).
Post-translational modification Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
Target Relevance information above includes information from UniProt accession: P29460
The UniProt Consortium

Data

Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).

ELISA with Human IL-12B/p40/NKSF2 Protein

Immobilized Human IL-12B, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test).

Immobilized Human IL-12B, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.26µg/ml determined by ELISA.

SDS-PAGE gel of Human IL-12B/p40/NKSF2 Protein

Human IL-12B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.